A vesicle microrheometer for high-throughput viscosity measurements of lipid and polymer membranes

Viscosity is a key property of cell membranes that controls mobility of embedded proteins and membrane remodeling. Measuring it is challenging because existing approaches involve complex experimental designs and/or models, and the applicability of some methods is limited to specific systems and memb...

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Published in:Biophysical journal Vol. 121; no. 6; p. 910
Main Authors: Faizi, Hammad A, Dimova, Rumiana, Vlahovska, Petia M
Format: Journal Article
Language:English
Published: United States 15.03.2022
Subjects:
Lipids - chemistry
Membranes
Polymers
Unilamellar Liposomes - chemistry
Viscosity
ISSN:1542-0086, 1542-0086
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Abstract Viscosity is a key property of cell membranes that controls mobility of embedded proteins and membrane remodeling. Measuring it is challenging because existing approaches involve complex experimental designs and/or models, and the applicability of some methods is limited to specific systems and membrane compositions. As a result there is scarcity of systematic data, and the reported values for membrane viscosity vary by orders of magnitude for the same system. Here, we show how viscosity of membranes can be easily obtained from the transient deformation of giant unilamellar vesicles. The approach enables a noninvasive, probe-independent, and high-throughput measurement of the viscosity of membranes made of lipids or polymers with a wide range of compositions and phase state. Using this novel method, we have collected a significant amount of data that provides insights into the relation between membrane viscosity, composition, and structure.
AbstractList Viscosity is a key property of cell membranes that controls mobility of embedded proteins and membrane remodeling. Measuring it is challenging because existing approaches involve complex experimental designs and/or models, and the applicability of some methods is limited to specific systems and membrane compositions. As a result there is scarcity of systematic data, and the reported values for membrane viscosity vary by orders of magnitude for the same system. Here, we show how viscosity of membranes can be easily obtained from the transient deformation of giant unilamellar vesicles. The approach enables a noninvasive, probe-independent, and high-throughput measurement of the viscosity of membranes made of lipids or polymers with a wide range of compositions and phase state. Using this novel method, we have collected a significant amount of data that provides insights into the relation between membrane viscosity, composition, and structure.
Viscosity is a key property of cell membranes that controls mobility of embedded proteins and membrane remodeling. Measuring it is challenging because existing approaches involve complex experimental designs and/or models, and the applicability of some methods is limited to specific systems and membrane compositions. As a result there is scarcity of systematic data, and the reported values for membrane viscosity vary by orders of magnitude for the same system. Here, we show how viscosity of membranes can be easily obtained from the transient deformation of giant unilamellar vesicles. The approach enables a noninvasive, probe-independent, and high-throughput measurement of the viscosity of membranes made of lipids or polymers with a wide range of compositions and phase state. Using this novel method, we have collected a significant amount of data that provides insights into the relation between membrane viscosity, composition, and structure.Viscosity is a key property of cell membranes that controls mobility of embedded proteins and membrane remodeling. Measuring it is challenging because existing approaches involve complex experimental designs and/or models, and the applicability of some methods is limited to specific systems and membrane compositions. As a result there is scarcity of systematic data, and the reported values for membrane viscosity vary by orders of magnitude for the same system. Here, we show how viscosity of membranes can be easily obtained from the transient deformation of giant unilamellar vesicles. The approach enables a noninvasive, probe-independent, and high-throughput measurement of the viscosity of membranes made of lipids or polymers with a wide range of compositions and phase state. Using this novel method, we have collected a significant amount of data that provides insights into the relation between membrane viscosity, composition, and structure.
Author Vlahovska, Petia M
Dimova, Rumiana
Faizi, Hammad A
Author_xml – sequence: 1
  givenname: Hammad A
  surname: Faizi
  fullname: Faizi, Hammad A
  organization: Department of Mechanical Engineering, Northwestern University, Evanston, Illinois
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  givenname: Rumiana
  surname: Dimova
  fullname: Dimova, Rumiana
  organization: Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, Science Park Golm, Potsdam, Germany
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  givenname: Petia M
  surname: Vlahovska
  fullname: Vlahovska, Petia M
  email: petia.vlahovska@northwestern.edu
  organization: Department of Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, Illinois. Electronic address: petia.vlahovska@northwestern.edu
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SubjectTerms Lipids - chemistry
Membranes
Polymers
Unilamellar Liposomes - chemistry
Viscosity
Title A vesicle microrheometer for high-throughput viscosity measurements of lipid and polymer membranes
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