One-step RT-PCR for detection of Zika virus

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are tim...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical virology Vol. 43; no. 1; pp. 96 - 101
Main Authors: Faye, Oumar, Faye, Ousmane, Dupressoir, Anne, Weidmann, Manfred, Ndiaye, Mady, Alpha Sall, Amadou
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 01.09.2008
Elsevier Science
Subjects:
Allergy and Immunology
Biological and medical sciences
Detection
Diagnosis
Flavivirus
Fundamental and applied biological sciences. Psychology
Genetic Variation
Human viral diseases
Infectious Disease
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Viral - isolation & purification
RT-PCR
Sensitivity and Specificity
Viral diseases
Viral Envelope Proteins - genetics
Virology
Zika virus
Zika Virus - genetics
Zika Virus - isolation & purification
Zika Virus Infection - diagnosis
RNA
DTT
L-15
dNTPs
Zika virus
ZF
ZIKV
TE
RT-PCR
FBS
CRORA
Diagnosis
Detection
pfu
WHO Collaborating Center for Arboviruses and Viral Hemorrhagic Fever
ribonucleic acid
plaque-forming unit
dithiothreitol
Zika fever
fetal bovine serum
deoxynucleotide triphosphates
L-15 Leibovitz medium
Tris–EDTA
reverse transcriptase polymerase chain reaction
Microbiology
Reverse transcription polymerase chain reaction
Virology
ISSN:1386-6532, 1873-5967
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. To develop a one-step RT-PCR assay to detect ZIKV in human serum. An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Undefined-1
ObjectType-Feature-3
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2008.05.005