One-step RT-PCR for detection of Zika virus

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are tim...

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Veröffentlicht in:	Journal of clinical virology Jg. 43; H. 1; S. 96 - 101
Hauptverfasser:	Faye, Oumar, Faye, Ousmane, Dupressoir, Anne, Weidmann, Manfred, Ndiaye, Mady, Alpha Sall, Amadou
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Amsterdam Elsevier B.V 01.09.2008 Elsevier Science
Schlagworte:	Allergy and Immunology Biological and medical sciences Detection Diagnosis Flavivirus Fundamental and applied biological sciences. Psychology Genetic Variation Human viral diseases Infectious Disease Infectious diseases Medical sciences Microbiology Miscellaneous Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - isolation & purification RT-PCR Sensitivity and Specificity Viral diseases Viral Envelope Proteins - genetics Virology Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus Infection - diagnosis RNA DTT L-15 dNTPs Zika virus ZF ZIKV TE RT-PCR FBS CRORA Diagnosis Detection pfu WHO Collaborating Center for Arboviruses and Viral Hemorrhagic Fever ribonucleic acid plaque-forming unit dithiothreitol Zika fever fetal bovine serum deoxynucleotide triphosphates L-15 Leibovitz medium Tris–EDTA reverse transcriptase polymerase chain reaction Microbiology Reverse transcription polymerase chain reaction Virology
ISSN:	1386-6532, 1873-5967
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Zusammenfassung:	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. To develop a one-step RT-PCR assay to detect ZIKV in human serum. An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Undefined-1 ObjectType-Feature-3
ISSN:	1386-6532 1873-5967
DOI:	10.1016/j.jcv.2008.05.005