Maximizing RNA yield from archival renal tumors and optimizing gene expression analysis

Formalin-fixed, paraffin-embedded tissues are widely available for gene expression analysis using TaqMan PCR. Five methods, including 4 commercial kits, for recovering RNA from paraffin-embedded renal tumor tissue were compared. The MasterPure kit from Epicentre produced the highest RNA yield. Howev...

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Bibliographic Details

Published in:	Journal of biomolecular screening Vol. 15; no. 1; p. 80
Main Authors:	Glenn, Sean T, Head, Karen L, Teh, Bin T, Gross, Kenneth W, Kim, Hyung L
Format:	Journal Article
Language:	English
Published:	United States 01.01.2010
Subjects:	Biological Specimen Banks Gene Expression Profiling - methods Gene Expression Regulation, Neoplastic Kidney Neoplasms - genetics Microfluidic Analytical Techniques Polymerase Chain Reaction - instrumentation Reagent Kits, Diagnostic Reverse Transcription - genetics RNA - metabolism RNA, Neoplasm - isolation & purification Taq Polymerase - metabolism
ISSN:	1552-454X, 1552-454X
Online Access:	Get more information
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