A structural analysis of M protein in coronavirus assembly and morphology

The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, t...

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Veröffentlicht in:Journal of structural biology Jg. 174; H. 1; S. 11 - 22
Hauptverfasser: Neuman, Benjamin W., Kiss, Gabriella, Kunding, Andreas H., Bhella, David, Baksh, M. Fazil, Connelly, Stephen, Droese, Ben, Klaus, Joseph P., Makino, Shinji, Sawicki, Stanley G., Siddell, Stuart G., Stamou, Dimitrios G., Wilson, Ian A., Kuhn, Peter, Buchmeier, Michael J.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Elsevier Inc 01.04.2011
Schlagworte:
Cell Line
Coronavirus
Coronavirus - metabolism
Coronavirus - ultrastructure
Coronavirus M Proteins
Cryo-electron microscopy
Cryo-electron tomography
Cryoelectron Microscopy
Electron Microscope Tomography
Humans
Pleomorphic virus structure
Viral matrix protein
Viral Matrix Proteins - ultrastructure
Virus Assembly - physiology
Virus Assembly - radiation effects
Viral matrix protein
Cryo-electron tomography
Coronavirus
Pleomorphic virus structure
Cryo-electron microscopy
ISSN:1047-8477, 1095-8657, 1095-8657
Online-Zugang:Volltext
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Zusammenfassung:The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1047-8477
1095-8657
1095-8657
DOI:10.1016/j.jsb.2010.11.021