Novel proteolytic activation of Ebolavirus glycoprotein GP by TMPRSS2 and cathepsin L at an uncharted position can compensate for furin cleavage

•The necessity of EBOV GP cleavage at the furin cleavage site was and is a subject of debate.•The furin cleavage site mutant EBOV GP_AGTAA, which was described as non-cleavable, is shown to be cleaved by TMPRSS2 and cathepsin L.•IF suggests that TMPRSS2 may cleave EBOV GP upon entry in the late endo...

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