Time-resolved Förster-resonance-energy-transfer DNA assay on an active CMOS microarray

We present an active oligonucleotide microarray platform for time-resolved Förster-resonance-energy-transfer (TR-FRET) assays. In these assays, immobilized probe is labeled with a donor fluorophore and analyte target is labeled with a fluorescence quencher. Changes in the fluorescence decay lifetime...

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Vydané v:Biosensors & bioelectronics Ročník 24; číslo 3; s. 383 - 390
Hlavní autori: Schwartz, David Eric, Gong, Ping, Shepard, Kenneth L.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Kidlington Elsevier B.V 15.11.2008
Elsevier
Predmet:
Active microarray
Biological and medical sciences
Biotechnology
DNA - analysis
Fluorescence Resonance Energy Transfer - methods
Fundamental and applied biological sciences. Psychology
Oligonucleotide Array Sequence Analysis - instrumentation
Oligonucleotide Array Sequence Analysis - methods
Oligonucleotide microarray
Quantum Dots
Semiconductors
Single-photon avalanche diode
SPAD array
TR-FRET
Active microarray
Single-photon avalanche diode
Quantum dots
Oligonucleotide microarray
TR-FRET
SPAD array
Quantum dot
Resonance energy transfer
Diode
DNA chip
Microarray
Array
Resonant energy transfer
DNA
ISSN:0956-5663, 1873-4235, 1873-4235
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Popis
Shrnutí:We present an active oligonucleotide microarray platform for time-resolved Förster-resonance-energy-transfer (TR-FRET) assays. In these assays, immobilized probe is labeled with a donor fluorophore and analyte target is labeled with a fluorescence quencher. Changes in the fluorescence decay lifetime of the donor are measured to determine the extent of hybridization. In this work, we demonstrate that TR-FRET assays have reduced sensitivity to variances in probe surface density compared with standard fluorescence-based microarray assays. Use of an active array substrate, fabricated in a standard complementary metal-oxide-semiconductor (CMOS) process, provides the additional benefits of reduced system complexity and cost. The array consists of 4096 independent single-photon avalanche diode (SPAD) pixel sites and features on-chip time-to-digital conversion. We demonstrate the functionality of our system by measuring a DNA target concentration series using TR-FRET with semiconductor quantum dot donors.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/j.bios.2008.04.015