Enterococcus species distribution among human and animal hosts using multiplex PCR

This study evaluated the use of Enterococcus species differentiation as a tool for microbial source tracking (MST) in recreational waters. Avian, mammalian and human faecal samples were screened for the occurrence of Enterococcus avium, Enterococcus casseliflavus, Enterococcus durans, Enterococcus g...

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Bibliographic Details
Published in:Journal of applied microbiology Vol. 109; no. 2; pp. 539 - 547
Main Authors: Layton, B.A, Walters, S.P, Lam, L.H, Boehm, A.B
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.08.2010
Blackwell Publishing Ltd
Blackwell
Oxford University Press
Subjects:
Biological and medical sciences
birds
classification
Enterococcus
Enterococcus - classification
Enterococcus - genetics
Enterococcus - isolation & purification
Enterococcus avium
Enterococcus casseliflavus
Enterococcus durans
Enterococcus faecalis
Enterococcus faecium
Enterococcus gallinarum
Enterococcus hirae
Enterococcus saccharolyticus
environmental
Environmental Monitoring
Environmental Monitoring - methods
environmental/recreational water
feces
Feces - microbiology
Fundamental and applied biological sciences. Psychology
genetics
Geographical distribution
host specificity
hosts
Humans
indicators
isolation & purification
Mammals
methods
microbial contamination
Microbiology
Microorganisms
Multiplexing
pollution
Pollution sources
polymerase chain reaction
Polymerase Chain Reaction - methods
Recreation
Recreational waters
Species
Water
Enterococcus
Human
environmental
Applied microbiology
microbial contamination
indicators
Polymerase chain reaction
Streptococcaceae
environmental/recreational water
Animal
Distribution
Bacteria
Micrococcales
Biological contamination
ISSN:1364-5072, 1365-2672, 1365-2672
Online Access:Get full text
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Summary:This study evaluated the use of Enterococcus species differentiation as a tool for microbial source tracking (MST) in recreational waters. Avian, mammalian and human faecal samples were screened for the occurrence of Enterococcus avium, Enterococcus casseliflavus, Enterococcus durans, Enterococcus gallinarum, Enterococcus faecium, Enterococcus faecalis, Enterococcus hirae and Enterococcus saccharolyticus using multiplex PCR. Host-specific patterns of Enterococcus species presence were observed only when data for multiple Enterococcus species were considered in aggregate. The results suggest that no single Enterococcus species is a reliable indicator of the host faecal source. However, Enterococcus species composite 'fingerprints' may offer auxiliary evidence for bacterial source identification. This study presents novel information on the enterococci species assemblages present in avian and mammalian hosts proximate to the nearshore ocean. These data will aid the development of appropriate MST strategies, and the approach used in this study could potentially assist in the identification of faecal pollution sources.
Bibliography:http://dx.doi.org/10.1111/j.1365-2672.2010.04675.x
L. H. Lam, Department of Microbiology & Immunology, Stanford University, Stanford, CA 94305, USA.
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ISSN:1364-5072
1365-2672
1365-2672
DOI:10.1111/j.1365-2672.2010.04675.x