Enterococcus species distribution among human and animal hosts using multiplex PCR

This study evaluated the use of Enterococcus species differentiation as a tool for microbial source tracking (MST) in recreational waters. Avian, mammalian and human faecal samples were screened for the occurrence of Enterococcus avium, Enterococcus casseliflavus, Enterococcus durans, Enterococcus g...

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Vydáno v:	Journal of applied microbiology Ročník 109; číslo 2; s. 539 - 547
Hlavní autoři:	Layton, B.A, Walters, S.P, Lam, L.H, Boehm, A.B
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.08.2010 Blackwell Publishing Ltd Blackwell Oxford University Press
Témata:	Biological and medical sciences birds classification Enterococcus Enterococcus - classification Enterococcus - genetics Enterococcus - isolation & purification Enterococcus avium Enterococcus casseliflavus Enterococcus durans Enterococcus faecalis Enterococcus faecium Enterococcus gallinarum Enterococcus hirae Enterococcus saccharolyticus environmental Environmental Monitoring Environmental Monitoring - methods environmental/recreational water feces Feces - microbiology Fundamental and applied biological sciences. Psychology genetics Geographical distribution host specificity hosts Humans indicators isolation & purification Mammals methods microbial contamination Microbiology Microorganisms Multiplexing pollution Pollution sources polymerase chain reaction Polymerase Chain Reaction - methods Recreation Recreational waters Species Water Enterococcus Human environmental Applied microbiology microbial contamination indicators Polymerase chain reaction Streptococcaceae environmental/recreational water Animal Distribution Bacteria Micrococcales Biological contamination
ISSN:	1364-5072, 1365-2672, 1365-2672
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Popis
Shrnutí:	This study evaluated the use of Enterococcus species differentiation as a tool for microbial source tracking (MST) in recreational waters. Avian, mammalian and human faecal samples were screened for the occurrence of Enterococcus avium, Enterococcus casseliflavus, Enterococcus durans, Enterococcus gallinarum, Enterococcus faecium, Enterococcus faecalis, Enterococcus hirae and Enterococcus saccharolyticus using multiplex PCR. Host-specific patterns of Enterococcus species presence were observed only when data for multiple Enterococcus species were considered in aggregate. The results suggest that no single Enterococcus species is a reliable indicator of the host faecal source. However, Enterococcus species composite 'fingerprints' may offer auxiliary evidence for bacterial source identification. This study presents novel information on the enterococci species assemblages present in avian and mammalian hosts proximate to the nearshore ocean. These data will aid the development of appropriate MST strategies, and the approach used in this study could potentially assist in the identification of faecal pollution sources.
Bibliografie:	http://dx.doi.org/10.1111/j.1365-2672.2010.04675.x L. H. Lam, Department of Microbiology & Immunology, Stanford University, Stanford, CA 94305, USA. Present address ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23
ISSN:	1364-5072 1365-2672 1365-2672
DOI:	10.1111/j.1365-2672.2010.04675.x