Rapid determination and continuous monitoring of propofol in microliter whole blood sample during anesthesia by paper spray ionization-mass spectrometry

Propofol is a widely used intravenous anesthetic agent in sedation and general anesthesia. To improve the safety and maintain the depth of anesthesia, it is important to develop a rapid, sensitive, and reliable method to monitor the concentration of propofol in blood during anesthesia continuously....

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry Vol. 413; no. 1; pp. 279 - 287
Main Authors: Liu, Ying, Zhang, Xiao-Hui, Mi, Wei-Dong, Zhou, Ying-Lin, Zhang, Chang-Sheng, Zhang, Xin-Xiang
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2021
Springer
Springer Nature B.V
Subjects:
Analysis
Analysis and chemistry
Analytical Chemistry
Anesthesia
anesthetics
Biochemistry
Blood
Blood levels
blood sampling
Characterization and Evaluation of Materials
Chemical precipitation
Chemistry
Chemistry and Materials Science
depth of anesthesia
detection limit
Food Science
Intravenous administration
intravenous injection
Ionization
Laboratory Medicine
Mass spectrometry
Mass spectroscopy
Methanol
Methods
Monitoring
Monitoring methods
Monitoring/Environmental Analysis
Observations
Propofol
Proteins
Research Paper
Scientific imaging
sedation
solvents
Spectroscopy
China
Whole blood
Ambient ionization mass spectrometry
Propofol
Monitoring in anesthesia
Protein micro-precipitation
ISSN:1618-2642, 1618-2650, 1618-2650
Online Access:Get full text
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Summary:Propofol is a widely used intravenous anesthetic agent in sedation and general anesthesia. To improve the safety and maintain the depth of anesthesia, it is important to develop a rapid, sensitive, and reliable method to monitor the concentration of propofol in blood during anesthesia continuously. Here, we present a novel strategy based on paper spray ionization-mass spectrometry (PSI-MS) to detect propofol. Samples (in 10 μL) were mixed with methanol as protein precipitation solvent and 2,6-dimethylphenol as internal standard. Protein micro-precipitation was achieved with methanol by vortexing and centrifuging for 5 s each, and propofol was extracted to the supernatant. PSI-MS was performed in negative ionization mode, and MS signal lasted for 1 min. The analysis of a single sample was completed within 2 min. The area ratios of propofol to internal standard were calculated for quantification. Limit of detection of 5.5 ng mL −1 and limit of quantification of 18.2 ng mL −1 were achieved for propofol in whole blood. Calibration curve was linear in the range of 0.02–10 μg mL −1 . The developed method was used successfully in monitoring the propofol concentration in 3 patients’ whole blood during anesthesia, showing its further application in controlling and feeding-back target concentration infusion. Graphical abstract
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ISSN:1618-2642
1618-2650
1618-2650
DOI:10.1007/s00216-020-02999-6