Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131

There is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species ( Leptonychotes weddellii , Mirounga leonina and Arctocephalus gazella ) from the west coast of the Antartic Peninsula were an...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Scientific reports Jg. 8; H. 1; S. 4678 - 11
Hauptverfasser: Mora, Azucena, García-Peña, Francisco Javier, Alonso, María Pilar, Pedraza-Diaz, Susana, Ortega-Mora, Luis Miguel, Garcia-Parraga, Daniel, López, Cecilia, Viso, Susana, Dahbi, Ghizlane, Marzoa, Juan, Sergeant, Martin J., García, Vanesa, Blanco, Jorge
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 16.03.2018
Nature Publishing Group
Schlagworte:
45
45/22
45/29
45/41
45/47
45/77
631/326/421
692/699
Animals
Antarctic Regions
Antarctic zone
Bacteremia
Bacterial Typing Techniques - methods
Caniformia - microbiology
DNA, Bacterial - genetics
E coli
Ecosystem
Escherichia coli
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Genomes
Humanities and Social Sciences
Humans
Microorganisms
Molecular Epidemiology
Molecular Typing
multidisciplinary
Phylogeny
Plasmids
Science
Science (multidisciplinary)
Antarctic Regions
ISSN:2045-2322, 2045-2322
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:There is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species ( Leptonychotes weddellii , Mirounga leonina and Arctocephalus gazella ) from the west coast of the Antartic Peninsula were analysed for the presence of Escherichia spp. with the recovery of 158 E . coli and three E . albertii isolates. From those, 23 harboured different eae variants (α1, β1, β2, ε1, θ1, κ, ο), including a bfpA -positive isolate (O49:H10-A-ST206, eae -k) classified as typical enteropathogenic E . coli . Noteworthy, 62 of the 158 E . coli isolates (39.2%) exhibited the ExPEC status and 27 (17.1%) belonged to sequence types (ST) frequently occurring among urinary/bacteremia ExPEC clones: ST12, ST73, ST95, ST131 and ST141. We found similarities >85% within the PFGE-macrorrestriction profiles of pinniped and human clinic O2:H6-B2-ST141 and O16:H5/O25b:H4-B2-ST131 isolates. The in silico analysis of ST131 Cplx genomes from the three pinnipeds (five O25:H4-ST131/PST43- fimH 22-virotype D; one O16:H5-ST131/PST506- fimH 41; one O25:H4-ST6252/PST9- fimH 22-virotype D1) identified IncF and IncI1 plasmids and revealed high core-genome similarities between pinniped and human isolates ( H 22 and H 41 subclones). This is the first study to demonstrate the worrisome presence of human-associated E . coli clonal groups, including ST131, in Antarctic pinnipeds.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-22943-0