The membrane-bound NAC transcription factor ANAC013 functions in mitochondrial retrograde regulation of the oxidative stress response in Arabidopsis

Upon disturbance of their function by stress, mitochondria can signal to the nucleus to steer the expression of responsive genes. This mitochondria-to-nucleus communication is often referred to as mitochondrial retrograde regulation (MRR). Although reactive oxygen species and calcium are likely cand...

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Published in:The Plant cell Vol. 25; no. 9; p. 3472
Main Authors: De Clercq, Inge, Vermeirssen, Vanessa, Van Aken, Olivier, Vandepoele, Klaas, Murcha, Monika W, Law, Simon R, Inzé, Annelies, Ng, Sophia, Ivanova, Aneta, Rombaut, Debbie, van de Cotte, Brigitte, Jaspers, Pinja, Van de Peer, Yves, Kangasjärvi, Jaakko, Whelan, James, Van Breusegem, Frank
Format: Journal Article
Language:English
Published: England 01.09.2013
Subjects:
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Binding Sites
Cell Nucleus - metabolism
Endoplasmic Reticulum - metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Mitochondria - metabolism
Mutation
Oligonucleotide Array Sequence Analysis
Oxidative Stress
Paraquat - pharmacology
Plants, Genetically Modified
Promoter Regions, Genetic - genetics
Protein Binding
Regulatory Sequences, Nucleic Acid - genetics
Rotenone - pharmacology
Seedlings - drug effects
Seedlings - genetics
Seedlings - physiology
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptional Activation
ISSN:1532-298X, 1532-298X
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Summary:Upon disturbance of their function by stress, mitochondria can signal to the nucleus to steer the expression of responsive genes. This mitochondria-to-nucleus communication is often referred to as mitochondrial retrograde regulation (MRR). Although reactive oxygen species and calcium are likely candidate signaling molecules for MRR, the protein signaling components in plants remain largely unknown. Through meta-analysis of transcriptome data, we detected a set of genes that are common and robust targets of MRR and used them as a bait to identify its transcriptional regulators. In the upstream regions of these mitochondrial dysfunction stimulon (MDS) genes, we found a cis-regulatory element, the mitochondrial dysfunction motif (MDM), which is necessary and sufficient for gene expression under various mitochondrial perturbation conditions. Yeast one-hybrid analysis and electrophoretic mobility shift assays revealed that the transmembrane domain-containing no apical meristem/Arabidopsis transcription activation factor/cup-shaped cotyledon transcription factors (ANAC013, ANAC016, ANAC017, ANAC053, and ANAC078) bound to the MDM cis-regulatory element. We demonstrate that ANAC013 mediates MRR-induced expression of the MDS genes by direct interaction with the MDM cis-regulatory element and triggers increased oxidative stress tolerance. In conclusion, we characterized ANAC013 as a regulator of MRR upon stress in Arabidopsis thaliana.
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ISSN:1532-298X
1532-298X
DOI:10.1105/tpc.113.117168