Discovery of a unique novel clade of mosquito-associated bunyaviruses

Bunyaviruses are the largest known family of RNA viruses, infecting vertebrates, insects, and plants. Here we isolated three novel bunyaviruses from mosquitoes sampled in Côte d'Ivoire, Ghana, and Uganda. The viruses define a highly diversified monophyletic sister clade to all members of the ge...

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Vydáno v:Journal of virology Ročník 87; číslo 23; s. 12850
Hlavní autoři: Marklewitz, Marco, Zirkel, Florian, Rwego, Innocent B, Heidemann, Hanna, Trippner, Pascal, Kurth, Andreas, Kallies, René, Briese, Thomas, Lipkin, W Ian, Drosten, Christian, Gillespie, Thomas R, Junglen, Sandra
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.12.2013
Témata:
Amino Acid Sequence
Animals
Base Sequence
Bunyaviridae - classification
Bunyaviridae - genetics
Bunyaviridae - growth & development
Bunyaviridae - isolation & purification
Bunyaviridae Infections - veterinary
Bunyaviridae Infections - virology
Cell Line
Culicidae - virology
Genome, Viral
Humans
Insect Vectors - virology
Mice
Molecular Sequence Data
Phylogeny
Sequence Alignment
Viral Proteins - chemistry
Viral Proteins - genetics
ISSN:1098-5514, 1098-5514
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Shrnutí:Bunyaviruses are the largest known family of RNA viruses, infecting vertebrates, insects, and plants. Here we isolated three novel bunyaviruses from mosquitoes sampled in Côte d'Ivoire, Ghana, and Uganda. The viruses define a highly diversified monophyletic sister clade to all members of the genus Orthobunyavirus and are virtually equidistant to orthobunyaviruses and tospoviruses. Maximal amino acid identities between homologous putative proteins of the novel group and orthobunyaviruses ranged between 12 and 25%. The type isolates, tentatively named Herbert virus (HEBV), Taï virus (TAIV), and Kibale virus (KIBV), comprised genomes with L, M, and S segments of about 7.4 kb, 2.7 kb, and 1.1 kb, respectively. HEBV, TAIV, and KIBV encode the shortest bunyavirus M segments known and did not seem to encode NSs and NSm proteins but contained an elongated L segment with an ∼500-nucleotide (nt) insertion that shows no identity to other bunyaviruses. The viruses replicated to high titers in insect cells but did not replicate in vertebrate cells. The enveloped virions were 90 to 110 nm in diameter and budded at cellular membranes with morphological features typical of the Golgi complex. Viral RNA recovered from infected cells showed 5'-terminal nontemplated sequences of 9 to 22 nt, suggestive of cap snatching during mRNA synthesis, as described for other bunyaviruses. Northern blotting identified RNA species of full and reduced lengths, suggested upon analogy with other bunyaviruses to constitute antigenomic-sense cRNA and transcript mRNAs, respectively. Functional studies will be necessary to determine if this group of viruses constitutes a novel genus in the bunyavirus family.
Bibliografie:ObjectType-Article-1
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ISSN:1098-5514
1098-5514
DOI:10.1128/JVI.01862-13