Salivary Biomarkers for Detection of Oral Squamous Cell Carcinoma in a Taiwanese Population

This study evaluated the discriminatory power of salivary transcriptomic and proteomic biomarkers in distinguishing oral squamous cell carcinoma cases from controls and potentially malignant oral disorders (PMOD). A total of 180 samples (60 OSCC patients, 60 controls, and 60 PMOD patients) were used...

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Vydáno v:Clinical cancer research Ročník 22; číslo 13; s. 3340 - 3347
Hlavní autoři: Gleber-Netto, Frederico Omar, Yakob, Maha, Li, Feng, Feng, Ziding, Dai, Jianliang, Kao, Huang-Kai, Chang, Yu-Liang, Chang, Kai-Ping, Wong, David T W
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.07.2016
Témata:
Adult
Aged
Biomarkers, Tumor - chemistry
Carcinoma, Squamous Cell - diagnosis
Dual Specificity Phosphatase 1 - analysis
Early Detection of Cancer - methods
Enzyme-Linked Immunosorbent Assay
Female
Head and Neck Neoplasms - diagnosis
Humans
Interleukin-1beta - analysis
Interleukin-8 - analysis
Male
Middle Aged
Mouth Neoplasms - diagnosis
Proteomics - methods
Risk Factors
Saliva - chemistry
Squamous Cell Carcinoma of Head and Neck
Taiwan
Taiwan
ISSN:1078-0432, 1557-3265
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Shrnutí:This study evaluated the discriminatory power of salivary transcriptomic and proteomic biomarkers in distinguishing oral squamous cell carcinoma cases from controls and potentially malignant oral disorders (PMOD). A total of 180 samples (60 OSCC patients, 60 controls, and 60 PMOD patients) were used in the study. Seven transcriptomic markers (IL8, IL1β, SAT1, OAZ1, DUSP1, S100P, and H3F3A) were measured using qPCR, and two proteomic markers (IL8 and IL1β) were evaluated by ELISA. Among 7 transcriptomic markers, transcript level of DUSP1 was significantly lower in OSCC patients than in controls and PMOD patients. Between the proteomic markers, the protein concentration of IL8 and IL1β was significantly higher in OSCC patients than controls and dysplasia patients. Univariate fractional polynomial (FP) models revealed that salivary IL8 protein (IL8p) has the highest AUC value between OSCC patients and controls (0.74) and between OSCC and PMOD patients (0.72). Applying a 2-marker FP model, salivary IL8p combined with IL1β gave the best AUC value for discrimination between OSCC patients and controls, as well as the IL8p combined with H3F3A mRNA, which gave the best AUC value for discrimination between OSCC and PMOD patients. Multivariate models analysis combining salivary analytes and risk factor exposure related to oral carcinogenesis formed the best combinatory variables for differentiation between OSCC versus PMOL (AUC = 0.80), OSCC versus controls (AUC = 0.87), and PMOD versus controls (AUC = 0.78). The combination of transcriptomic and proteomic salivary markers is of great value for oral cancer detection and differentiation from PMOD patients and controls. Clin Cancer Res; 22(13); 3340-7. ©2016 AACR.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1078-0432
1557-3265
DOI:10.1158/1078-0432.CCR-15-1761