Protein microarrays with carbon nanotubes as multicolor Raman labels

The picomolar sensitivity of fluorescence-based protein detection limits the use of protein arrays in research and clinical diagnosis. Chen et al . use antibody-tagged single-walled carbon nanotubes as multicolor Raman labels to detect femtomolar levels of serum analytes over a wide dynamic range. T...

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Veröffentlicht in:	Nature biotechnology Jg. 26; H. 11; S. 1285 - 1292
Hauptverfasser:	Chen, Zhuo, Tabakman, Scott M, Goodwin, Andrew P, Kattah, Michael G, Daranciang, Dan, Wang, Xinran, Zhang, Guangyu, Li, Xiaolin, Liu, Zhuang, Utz, Paul J, Jiang, Kaili, Fan, Shoushan, Dai, Hongjie
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	New York Nature Publishing Group US 01.11.2008 Nature Publishing Group
Schlagworte:	Agriculture Animals Autoantibodies - blood Autoimmune diseases Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Carbon Carbon Isotopes - chemistry Carbon Radioisotopes - chemistry Detection limits Fluorescence Fundamental and applied biological sciences. Psychology Granulomatosis with Polyangiitis - diagnosis Granulomatosis with Polyangiitis - immunology Health. Pharmaceutical industry Humans Identification and classification Industrial applications and implications. Economical aspects Life Sciences Methods Mice Miscellaneous Myeloblastin - immunology Nanotechnology Nanotubes Nanotubes, Carbon - chemistry Photobleaching Protein Array Analysis - methods Protein microarrays Proteins Research methodology Signal to noise ratio Spectrum Analysis, Raman - methods United States Human Nanotube Autoantibody Biological marker Wegener granulomatosis Autoimmune disease Protein microarray Carbon Protein Raman scattering Serum Diagnosis Detection
ISSN:	1087-0156, 1546-1696, 1546-1696
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Zusammenfassung:	The picomolar sensitivity of fluorescence-based protein detection limits the use of protein arrays in research and clinical diagnosis. Chen et al . use antibody-tagged single-walled carbon nanotubes as multicolor Raman labels to detect femtomolar levels of serum analytes over a wide dynamic range. The current sensitivity of standard fluorescence-based protein detection limits the use of protein arrays in research and clinical diagnosis. Here, we use functionalized, macromolecular single-walled carbon nanotubes (SWNTs) as multicolor Raman labels for highly sensitive, multiplexed protein detection in an arrayed format. Unlike fluorescence methods, Raman detection benefits from the sharp scattering peaks of SWNTs with minimal background interference, affording a high signal-to-noise ratio needed for ultra-sensitive detection. When combined with surface-enhanced Raman scattering substrates, the strong Raman intensity of SWNT tags affords protein detection sensitivity in sandwich assays down to 1 fM—a three-order-of-magnitude improvement over most reports of fluorescence-based detection. We use SWNT Raman tags to detect human autoantibodies against proteinase 3, a biomarker for the autoimmune disease Wegener's granulomatosis, diluted up to 10 7 -fold in 1% human serum. SWNT Raman tags are not subject to photobleaching or quenching. By conjugating different antibodies to pure 12 C and 13 C SWNT isotopes, we demonstrate multiplexed two-color SWNT Raman-based protein detection.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 14 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Undefined-1 ObjectType-Feature-3
ISSN:	1087-0156 1546-1696 1546-1696
DOI:	10.1038/nbt.1501