Protein microarrays with carbon nanotubes as multicolor Raman labels

The picomolar sensitivity of fluorescence-based protein detection limits the use of protein arrays in research and clinical diagnosis. Chen et al . use antibody-tagged single-walled carbon nanotubes as multicolor Raman labels to detect femtomolar levels of serum analytes over a wide dynamic range. T...

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Bibliographic Details
Published in:Nature biotechnology Vol. 26; no. 11; pp. 1285 - 1292
Main Authors: Chen, Zhuo, Tabakman, Scott M, Goodwin, Andrew P, Kattah, Michael G, Daranciang, Dan, Wang, Xinran, Zhang, Guangyu, Li, Xiaolin, Liu, Zhuang, Utz, Paul J, Jiang, Kaili, Fan, Shoushan, Dai, Hongjie
Format: Journal Article
Language:English
Published: New York Nature Publishing Group US 01.11.2008
Nature Publishing Group
Subjects:
Agriculture
Animals
Autoantibodies - blood
Autoimmune diseases
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Carbon
Carbon Isotopes - chemistry
Carbon Radioisotopes - chemistry
Detection limits
Fluorescence
Fundamental and applied biological sciences. Psychology
Granulomatosis with Polyangiitis - diagnosis
Granulomatosis with Polyangiitis - immunology
Health. Pharmaceutical industry
Humans
Identification and classification
Industrial applications and implications. Economical aspects
Life Sciences
Methods
Mice
Miscellaneous
Myeloblastin - immunology
Nanotechnology
Nanotubes
Nanotubes, Carbon - chemistry
Photobleaching
Protein Array Analysis - methods
Protein microarrays
Proteins
Research methodology
Signal to noise ratio
Spectrum Analysis, Raman - methods
United States
Human
Nanotube
Autoantibody
Biological marker
Wegener granulomatosis
Autoimmune disease
Protein microarray
Carbon
Protein
Raman scattering
Serum
Diagnosis
Detection
ISSN:1087-0156, 1546-1696, 1546-1696
Online Access:Get full text
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Summary:The picomolar sensitivity of fluorescence-based protein detection limits the use of protein arrays in research and clinical diagnosis. Chen et al . use antibody-tagged single-walled carbon nanotubes as multicolor Raman labels to detect femtomolar levels of serum analytes over a wide dynamic range. The current sensitivity of standard fluorescence-based protein detection limits the use of protein arrays in research and clinical diagnosis. Here, we use functionalized, macromolecular single-walled carbon nanotubes (SWNTs) as multicolor Raman labels for highly sensitive, multiplexed protein detection in an arrayed format. Unlike fluorescence methods, Raman detection benefits from the sharp scattering peaks of SWNTs with minimal background interference, affording a high signal-to-noise ratio needed for ultra-sensitive detection. When combined with surface-enhanced Raman scattering substrates, the strong Raman intensity of SWNT tags affords protein detection sensitivity in sandwich assays down to 1 fM—a three-order-of-magnitude improvement over most reports of fluorescence-based detection. We use SWNT Raman tags to detect human autoantibodies against proteinase 3, a biomarker for the autoimmune disease Wegener's granulomatosis, diluted up to 10 7 -fold in 1% human serum. SWNT Raman tags are not subject to photobleaching or quenching. By conjugating different antibodies to pure 12 C and 13 C SWNT isotopes, we demonstrate multiplexed two-color SWNT Raman-based protein detection.
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ISSN:1087-0156
1546-1696
1546-1696
DOI:10.1038/nbt.1501