Bilateral entry and release of Middle East respiratory syndrome coronavirus induces profound apoptosis of human bronchial epithelial cells

The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) infects human bronchial epithelial Calu-3 cells. Unlike severe acute respiratory syndrome (SARS)-CoV, which exclusively infects and releases through the apical route, this virus can do so through either side of polarized Calu-...

Full description

Saved in:
Bibliographic Details
Published in:Journal of virology Vol. 87; no. 17; p. 9953
Main Authors: Tao, Xinrong, Hill, Terence E, Morimoto, Chikao, Peters, Clarence J, Ksiazek, Thomas G, Tseng, Chien-Te K
Format: Journal Article
Language:English
Published: United States 01.09.2013
Subjects:
Apoptosis
Bronchi - pathology
Bronchi - virology
Cell Line
Cell Polarity
Coronavirus - pathogenicity
Coronavirus - physiology
Cytopathogenic Effect, Viral - physiology
Epithelial Cells - pathology
Epithelial Cells - virology
Humans
SARS Virus - pathogenicity
SARS Virus - physiology
Species Specificity
Virus Internalization
Virus Release - physiology
ISSN:1098-5514, 1098-5514
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) infects human bronchial epithelial Calu-3 cells. Unlike severe acute respiratory syndrome (SARS)-CoV, which exclusively infects and releases through the apical route, this virus can do so through either side of polarized Calu-3 cells. Infection results in profound apoptosis within 24 h irrespective of its production of titers that are lower than those of SARS-CoV. Together, our results provide new insights into the dissemination and pathogenesis of MERS-CoV and may indicate that the virus differs markedly from SARS-CoV.
AbstractList The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) infects human bronchial epithelial Calu-3 cells. Unlike severe acute respiratory syndrome (SARS)-CoV, which exclusively infects and releases through the apical route, this virus can do so through either side of polarized Calu-3 cells. Infection results in profound apoptosis within 24 h irrespective of its production of titers that are lower than those of SARS-CoV. Together, our results provide new insights into the dissemination and pathogenesis of MERS-CoV and may indicate that the virus differs markedly from SARS-CoV.
The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) infects human bronchial epithelial Calu-3 cells. Unlike severe acute respiratory syndrome (SARS)-CoV, which exclusively infects and releases through the apical route, this virus can do so through either side of polarized Calu-3 cells. Infection results in profound apoptosis within 24 h irrespective of its production of titers that are lower than those of SARS-CoV. Together, our results provide new insights into the dissemination and pathogenesis of MERS-CoV and may indicate that the virus differs markedly from SARS-CoV.The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) infects human bronchial epithelial Calu-3 cells. Unlike severe acute respiratory syndrome (SARS)-CoV, which exclusively infects and releases through the apical route, this virus can do so through either side of polarized Calu-3 cells. Infection results in profound apoptosis within 24 h irrespective of its production of titers that are lower than those of SARS-CoV. Together, our results provide new insights into the dissemination and pathogenesis of MERS-CoV and may indicate that the virus differs markedly from SARS-CoV.
Author Morimoto, Chikao
Tao, Xinrong
Peters, Clarence J
Tseng, Chien-Te K
Hill, Terence E
Ksiazek, Thomas G
Author_xml – sequence: 1
  givenname: Xinrong
  surname: Tao
  fullname: Tao, Xinrong
  organization: Departments of Microbiology and Immunologya and Center for Biodefense and Emerging Infectious Disease,c University of Texas Medical Branch, Galveston, Texas, USA
– sequence: 2
  givenname: Terence E
  surname: Hill
  fullname: Hill, Terence E
– sequence: 3
  givenname: Chikao
  surname: Morimoto
  fullname: Morimoto, Chikao
– sequence: 4
  givenname: Clarence J
  surname: Peters
  fullname: Peters, Clarence J
– sequence: 5
  givenname: Thomas G
  surname: Ksiazek
  fullname: Ksiazek, Thomas G
– sequence: 6
  givenname: Chien-Te K
  surname: Tseng
  fullname: Tseng, Chien-Te K
BackLink https://www.ncbi.nlm.nih.gov/pubmed/23824802$$D View this record in MEDLINE/PubMed
BookMark eNpNkMtOwzAQRS1URB-wY428ZJPiR15dQlWgqIgNsI0m8UQ1SuxgO0j9Bb6aRBSJ1VyNjs5czZxMjDVIyCVnS85FfvP0vl0ynqQi4vKEzDhb5VGS8HjyL0_J3PsPxngcp_EZmQqZizhnYka-73QDAR00FE1wBwpGUYcNgkdqa_qslWqQbsCHYe077SDYAfMHo5xtkVbWWQNf2vWeaqP6Cj3tnK1tP4igs12wXvtRte9bMLQc8Gqvx3udDntsxlhh0_hzclpD4_HiOBfk7X7zun6Mdi8P2_XtLqoSzkLERVIJxWq2EkpyVGlSpwKklDXjkoPKMo4yWbEyrVIsEViZxZiDBK7qNM9WYkGuf71Dzc8efSha7ccGYND2vuCxiKVIZZ4P6NUR7csWVdE53YI7FH__Ez8vmnZ9
CitedBy_id crossref_primary_10_1002_rmv_1792
crossref_primary_10_1128_JVI_00683_18
crossref_primary_10_1155_2021_8856018
crossref_primary_10_1093_infdis_jiv380
crossref_primary_10_1186_1471_2164_15_1161
crossref_primary_10_1016_j_virol_2015_01_020
crossref_primary_10_1016_j_virol_2016_10_005
crossref_primary_10_1128_JVI_03001_13
crossref_primary_10_3390_v14020231
crossref_primary_10_1089_vbz_2017_2191
crossref_primary_10_3390_v13071312
crossref_primary_10_1016_j_bbamcr_2023_119536
crossref_primary_10_1016_j_yexcr_2020_112204
crossref_primary_10_1016_j_vaccine_2014_08_086
crossref_primary_10_1128_JVI_00107_19
crossref_primary_10_1128_CMR_00102_14
crossref_primary_10_3390_cells9091963
crossref_primary_10_1016_j_jbc_2022_101584
crossref_primary_10_1016_j_jmb_2018_04_016
crossref_primary_10_1016_j_virol_2017_11_012
crossref_primary_10_1128_mSystems_00043_16
crossref_primary_10_1097_MCP_0000000000000046
crossref_primary_10_1016_j_vaccine_2014_02_004
crossref_primary_10_1186_s12985_016_0479_5
crossref_primary_10_1038_s42003_021_02250_7
crossref_primary_10_1590_0074_02760210127
crossref_primary_10_1016_j_ajpath_2015_09_014
crossref_primary_10_1007_s10930_023_10103_x
crossref_primary_10_1371_journal_pone_0081587
crossref_primary_10_1016_j_virusres_2014_11_013
crossref_primary_10_3390_cells10061291
crossref_primary_10_1016_j_antiviral_2016_06_003
crossref_primary_10_1073_pnas_1405889111
crossref_primary_10_1146_annurev_micro_020518_115759
crossref_primary_10_12688_f1000research_2_163_v1
crossref_primary_10_1186_s12929_024_01062_1
crossref_primary_10_1016_j_micinf_2018_03_005
crossref_primary_10_1128_JVI_00433_14
crossref_primary_10_1080_10408363_2020_1783198
crossref_primary_10_1038_cmi_2015_03
crossref_primary_10_3390_diseases4030026
crossref_primary_10_1038_s41586_022_05148_4
crossref_primary_10_3389_fpubh_2021_756049
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1128/JVI.01562-13
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Biology
EISSN 1098-5514
ExternalDocumentID 23824802
Genre Comparative Study
Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NIAID NIH HHS
  grantid: UC7 AI094660
– fundername: NIAID NIH HHS
  grantid: R01AI098775-01-PIS
– fundername: NIAID NIH HHS
  grantid: R01 AI098775
GroupedDBID ---
-~X
.55
.GJ
0R~
18M
29L
2WC
39C
3O-
4.4
41~
53G
5GY
5RE
5VS
6TJ
85S
AAFWJ
AAGFI
AAYJJ
ABPPZ
ACGFO
ACNCT
ADBBV
ADXHL
AENEX
AFFNX
AGVNZ
AI.
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
C1A
CGR
CS3
CUY
CVF
D0S
DIK
E3Z
EBS
ECM
EIF
EJD
F5P
FRP
GX1
H13
HYE
HZ~
IH2
KQ8
MVM
N9A
NPM
O9-
OHT
OK1
P2P
RHI
RNS
RPM
RSF
TR2
UPT
VH1
W2D
W8F
WH7
WOQ
X7M
Y6R
YQT
ZGI
ZXP
~02
~KM
7X8
AFPKN
ID FETCH-LOGICAL-c510t-125c2d0f092d31ed65f62a333f0131ad771e3590b6c6ebea0b74e8a3a1df68792
IEDL.DBID 7X8
ISICitedReferencesCount 49
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000323148500055&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 1098-5514
IngestDate Sun Nov 09 14:30:27 EST 2025
Mon Jul 21 06:05:49 EDT 2025
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 17
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c510t-125c2d0f092d31ed65f62a333f0131ad771e3590b6c6ebea0b74e8a3a1df68792
Notes ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/3754134
PMID 23824802
PQID 1424326388
PQPubID 23479
ParticipantIDs proquest_miscellaneous_1424326388
pubmed_primary_23824802
PublicationCentury 2000
PublicationDate 2013-09-01
PublicationDateYYYYMMDD 2013-09-01
PublicationDate_xml – month: 09
  year: 2013
  text: 2013-09-01
  day: 01
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Journal of virology
PublicationTitleAlternate J Virol
PublicationYear 2013
References 7937926 - Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9960-4
15577937 - Nat Med. 2004 Dec;10(12 Suppl):S88-97
23449793 - J Virol. 2013 May;87(9):5300-4
15258961 - J Med Virol. 2004 Sep;74(1):1-7
15731278 - J Virol. 2005 Mar;79(6):3846-50
23232719 - MBio. 2012;3(6). pii: e00515-12. doi: 10.1128/mBio.00515-12
23532101 - J Infect Dis. 2013 Jun 1;207(11):1743-52
10900005 - Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8439-44
23422412 - MBio. 2013;4(1):e00611-12
23631916 - MBio. 2013;4(3):e00165-13
23170002 - MBio. 2012;3(6). pii: e00473-12. doi: 10.1128/mBio.00473-12
17108019 - J Virol. 2007 Feb;81(3):1162-73
23486063 - Nature. 2013 Mar 14;495(7440):251-4
16014910 - J Virol. 2005 Aug;79(15):9470-9
23620378 - J Gen Virol. 2013 Aug;94(Pt 8):1749-60
17079315 - J Virol. 2007 Jan;81(2):813-21
15353589 - Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14186-91
23678167 - J Virol. 2013 Jul;87(14):7790-2
References_xml – reference: 17108019 - J Virol. 2007 Feb;81(3):1162-73
– reference: 16014910 - J Virol. 2005 Aug;79(15):9470-9
– reference: 7937926 - Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9960-4
– reference: 23486063 - Nature. 2013 Mar 14;495(7440):251-4
– reference: 23422412 - MBio. 2013;4(1):e00611-12
– reference: 23532101 - J Infect Dis. 2013 Jun 1;207(11):1743-52
– reference: 23631916 - MBio. 2013;4(3):e00165-13
– reference: 23449793 - J Virol. 2013 May;87(9):5300-4
– reference: 15577937 - Nat Med. 2004 Dec;10(12 Suppl):S88-97
– reference: 15353589 - Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14186-91
– reference: 15258961 - J Med Virol. 2004 Sep;74(1):1-7
– reference: 23232719 - MBio. 2012;3(6). pii: e00515-12. doi: 10.1128/mBio.00515-12
– reference: 23170002 - MBio. 2012;3(6). pii: e00473-12. doi: 10.1128/mBio.00473-12
– reference: 23620378 - J Gen Virol. 2013 Aug;94(Pt 8):1749-60
– reference: 17079315 - J Virol. 2007 Jan;81(2):813-21
– reference: 10900005 - Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8439-44
– reference: 23678167 - J Virol. 2013 Jul;87(14):7790-2
– reference: 15731278 - J Virol. 2005 Mar;79(6):3846-50
SSID ssj0014464
Score 2.3341
Snippet The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) infects human bronchial epithelial Calu-3 cells. Unlike severe acute respiratory...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
StartPage 9953
SubjectTerms Apoptosis
Bronchi - pathology
Bronchi - virology
Cell Line
Cell Polarity
Coronavirus - pathogenicity
Coronavirus - physiology
Cytopathogenic Effect, Viral - physiology
Epithelial Cells - pathology
Epithelial Cells - virology
Humans
SARS Virus - pathogenicity
SARS Virus - physiology
Species Specificity
Virus Internalization
Virus Release - physiology
Title Bilateral entry and release of Middle East respiratory syndrome coronavirus induces profound apoptosis of human bronchial epithelial cells
URI https://www.ncbi.nlm.nih.gov/pubmed/23824802
https://www.proquest.com/docview/1424326388
Volume 87
WOSCitedRecordID wos000323148500055&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LT9wwELZ4FIkLUEopr8pIXF0SOxs7J9QiECCx4kCrva0mfkgrrZJ0vSD1L_RXd8abLSckJC5RFDmJZc94PnseH2NnPrO51ToXde2kKEBpAQgEhHRVDsHnymV1IpvQw6EZjaqH_sAt9mGVyzUxLdSutXRGfk4ZWQg1lDEX3W9BrFHkXe0pNFbZukIoQ4qpRy9eBNzqJK8y1cwkZLAMfJfm_O7X7TfKIpaCiA1eA5fJyFxvv7d7O2yrh5f8-0IePrIV3-yyjQXh5J9P7O-PyRQo53jKE6MIh8Zxok1BW8bbwO_TcQW_gjjHx_-d8HxZ14BbKngAz5PZU-S4m0e5iJxov4mciUPXdvM2TiJ9KpH_8RqbUzQ1_q-j7I8p3ZKzIO6xn9dXj5c3omdjEBb1di4QCVnpspBV0qncu3IQSglKqUAle8DhhHs1qLK6tCVKBmS1LrwBBbkLpdGV_MzWmrbxXxgHWQwyACk1zpHVAS0i1KYsShtsVRp3wE6XgzxGaadeQePbpzh-GeYDtr-YqXG3KMsxRvAhC5PJwze8fcQ2ZeK1oGCxY7YeUNf9Cftgn-eTOPuaxAivw4f7fwUZ1V0
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Bilateral+entry+and+release+of+Middle+East+respiratory+syndrome+coronavirus+induces+profound+apoptosis+of+human+bronchial+epithelial+cells&rft.jtitle=Journal+of+virology&rft.au=Tao%2C+Xinrong&rft.au=Hill%2C+Terence+E&rft.au=Morimoto%2C+Chikao&rft.au=Peters%2C+Clarence+J&rft.date=2013-09-01&rft.eissn=1098-5514&rft.volume=87&rft.issue=17&rft.spage=9953&rft_id=info:doi/10.1128%2FJVI.01562-13&rft_id=info%3Apmid%2F23824802&rft_id=info%3Apmid%2F23824802&rft.externalDocID=23824802
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1098-5514&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1098-5514&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1098-5514&client=summon