Archaeal Diversity of Upland Rice Field Soils Assessed by the Terminal Restriction Fragment Length Polymorphism Method Combined with Real Time Quantitative-PCR and a Clone Library Analysis

The PCR amplification-based analysis of microbial diversity is subject to potential problems. In this study, to minimize the bias toward a 1:1 ratio in multitemplate PCR, a real-time PCR assay was carried out using a quenching fluorescence dye primer and amplification efficiency was monitored. Then...

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Vydané v:	Microbes and environments Ročník 23; číslo 3; s. 237
Hlavní autori:	Nishizawa, Tomoyasu, Komatsuzaki, Masakazu, Kaneko, Nobuhiro, Ohta, Hiroyuki
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Japan 01.01.2008
ISSN:	1342-6311
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