Archaeal Diversity of Upland Rice Field Soils Assessed by the Terminal Restriction Fragment Length Polymorphism Method Combined with Real Time Quantitative-PCR and a Clone Library Analysis

The PCR amplification-based analysis of microbial diversity is subject to potential problems. In this study, to minimize the bias toward a 1:1 ratio in multitemplate PCR, a real-time PCR assay was carried out using a quenching fluorescence dye primer and amplification efficiency was monitored. Then...

Full description

Bibliographic Details

Published in:	Microbes and environments Vol. 23; no. 3; p. 237
Main Authors:	Nishizawa, Tomoyasu, Komatsuzaki, Masakazu, Kaneko, Nobuhiro, Ohta, Hiroyuki
Format:	Journal Article
Language:	English
Published:	Japan 01.01.2008
ISSN:	1342-6311
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!