Smacoviridae: a new family of animal-associated single-stranded DNA viruses

Smacoviruses have small (∼2.3-2.9 kb), circular single-stranded DNA genomes encoding rolling circle replication-associated proteins (Rep) and unique capsid proteins. Although smacoviruses are prevalent in faecal matter of various vertebrates, including humans, none of these viruses have been culture...

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Veröffentlicht in:	Archives of virology Jg. 163; H. 7; S. 2005 - 2015
Hauptverfasser:	Varsani, Arvind, Krupovic, Mart
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Vienna Springer Vienna 01.07.2018 Springer Nature B.V Springer Verlag
Schlagworte:	Amino Acid Sequence amino acid sequences Animals Biodiversity Biomedical and Life Sciences Biomedicine circular DNA coat proteins Deoxyribonucleic acid DNA DNA Viruses DNA Viruses - classification DNA Viruses - genetics DNA Viruses - isolation & purification DNA, Circular DNA, Single-Stranded DNA, Single-Stranded - genetics DNA, Viral DNA, Viral - genetics Feces Feces - virology Geminiviridae Geminiviridae - genetics Genera genome Genome, Viral Genomes Humans Infectious Diseases Life Sciences Medical Microbiology Metagenomics Metagenomics - methods Microbiology and Parasitology new family New genera new genus Nucleotide sequence Phylogeny Sequence Analysis, DNA sequence diversity Single-stranded DNA Systematics, Phylogenetics and taxonomy Taxonomy Virology Virology Division News viruses
ISSN:	0304-8608, 1432-8798, 1432-8798
Online-Zugang:	Volltext
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Zusammenfassung:	Smacoviruses have small (∼2.3-2.9 kb), circular single-stranded DNA genomes encoding rolling circle replication-associated proteins (Rep) and unique capsid proteins. Although smacoviruses are prevalent in faecal matter of various vertebrates, including humans, none of these viruses have been cultured thus far. Smacoviruses display ∼45% genome-wide sequence diversity, which is very similar to that found within other families of circular Rep-encoding single-stranded (CRESS) DNA viruses, including members of the families Geminiviridae (46% diversity) and Genomoviridae (47% diversity). Here, we announce the creation of a new family Smacoviridae and describe a sequence-based taxonomic framework which was used to classify 83 smacovirus genomes into 43 species within six new genera, Bovismacovirus (n=3), Cosmacovirus (n=1), Dragsmacovirus (n=1), Drosmacovirus (n=3), Huchismacovirus (n=7), and Porprismacovirus (n=28). As in the case of genomoviruses, the species demarcation is based on the genome-wide pairwise identity, whereas genera are established based on the Rep amino acid sequence identity coupled with strong phylogenetic support. A similar sequence-based taxonomic framework should guide the classification of an astonishing diversity of other uncultured and currently unclassified CRESS DNA viruses discovered by metagenomic approaches.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0304-8608 1432-8798 1432-8798
DOI:	10.1007/s00705-018-3820-z