Green-Synthesized Silver Nanoparticles Using Filipendula ulmaria (L.) Maxim. and Salvia verticillata L. Extracts Inhibit Migration and Modulate Redox Homeostasis in Human Breast Cancer Cells via Nrf-2 Signaling Pathway

Breast cancer is a leading cancer diagnosis for women around the world, with a variable degree of curability. Conventional chemotherapeutic treatments often induce toxicity and damage to healthy tissues, as well as the development of drug resistance, which is why an increasing number of new therapeu...

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Published in:Antioxidants Vol. 14; no. 4; p. 469
Main Authors: Matić, Miloš, Obradović, Ana, Paunović, Milica, Ognjanović, Branka, Mihailović, Vladimir, Srećković, Nikola, Stanković, Milan
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 14.04.2025
MDPI
Subjects:
Acids
Antineoplastic drugs
Antioxidants
Antitumor activity
Breast cancer
Cancer
Cancer therapies
Cell cycle
Cell migration
Chemotherapy
Development and progression
Drug resistance
Enzymes
Estrogens
Fatalities
Filipendula ulmaria
Free radicals
GA-binding protein
Gelatinase B
Glutathione
Health aspects
Homeostasis
Lipid peroxidation
Matrix metalloproteinase
Metalloproteinase
Metastases
migration
MMP-9
Mortality
Nanoparticles
Natural products
Nitrites
Nrf-2
Physiology
redox homeostasis
Salvia verticillata
Signal transduction
Silver
silver nanoparticles
Superoxide
Superoxide anions
Toxicity
Tumors
United States
migration
breast cancer
redox homeostasis
silver nanoparticles
MMP-9
Nrf-2
ISSN:2076-3921, 2076-3921
Online Access:Get full text
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Summary:Breast cancer is a leading cancer diagnosis for women around the world, with a variable degree of curability. Conventional chemotherapeutic treatments often induce toxicity and damage to healthy tissues, as well as the development of drug resistance, which is why an increasing number of new therapeutic regimens focus on the use of natural products and various modifications of their delivery to target tissues. Silver nanoparticles possess unique physicochemical characteristics, notably their increased surface area, suggesting that they hold significant potential for biomedical applications. This research evaluates the capacity of silver nanoparticles green synthesized with aqueous extracts of Filipendula ulmaria (FUAgNPs) and Salvia verticillata (SVAgNPs) to alter migration and redox homeostasis in the human breast cancer cell line MDA-MB-231. To determine the values of redox homeostasis parameters, the cells were treated with five different concentrations (5, 10, 20, 50, and 100 μg/mL) for 24 h and 72 h, while to test the migratory potential and concentrations of matrix metalloproteinase-9 (MMP-9) and nuclear factor erythroid 2–related factor 2 (Nrf-2), the cells were treated at two concentrations (5 and 50 µg/mL) for 72 h. The obtained results indicate increased production of superoxide anion radicals, malondialdehyde (MDA), and nitrites after the investigated treatment on MDA-MB-231 cells. The treatments induced only a slight elevation in Nrf-2 levels, which correlates with weak de novo synthesis of reduced glutathione (GSH), suggesting that the tested nanoparticles weaken the inherent antioxidative systems of the tested cells. The migration potential of cells was significantly reduced, and MMP-9 concentration was significantly inhibited. Based on the demonstrated antitumor effect, confirmed by the reduced migratory potential of the examined cells and disrupted redox balance, these nanoparticles have potential for additional investigation with the aim of improving the efficacy of antitumor therapy. Also, FUAgNPs and SVAgNPs possess the capacity to be potentially promising novel chemotherapeutic agents against breast cancer progression and metastasis.
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ISSN:2076-3921
2076-3921
DOI:10.3390/antiox14040469