Flow cytometric analysis of hepatopancreatic cells from Armadillidium vulgare highlights terrestrial isopods as efficient environmental bioindicators in ex vivo settings

Several studies have reported the high bioindication capacity of Isopoda (Crustacea, Oniscidea), which is related to their important ability to accumulate contaminants, usefulness in soil ecotoxicology and bioindication activities. Any change in the isopod population, diversity and life cycle can in...

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Bibliographic Details
Published in:Environmental science and pollution research international Vol. 31; no. 6; pp. 9745 - 9763
Main Authors: Panza, Giovanna, Montanari, Mariele, Lopez, Daniele, Burattini, Sabrina, Ciacci, Caterina, Fumelli, Piermarco Paci, Pasini, Giovanni, Fusi, Vieri, Giorgi, Luca, Grandoni, Francesco, Papa, Stefano, Santolini, Riccardo, Canonico, Barbara
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2024
Springer Nature B.V
Subjects:
Aquatic Pollution
Armadillidium vulgare
Atmospheric Protection/Air Quality Control/Air Pollution
Biodiversity
Bioindicators
Biomarkers
Biomonitoring
Cell viability
Contaminants
Digestive system
Disaggregation
Earth and Environmental Science
ecological imbalance
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Environmental quality
Environmental science
Flow cytometry
Heavy metals
Hepatopancreas
histology
Indicator species
Investigations
Isopoda
Life cycles
Morphology
Organisms
Parameters
Pollution
Pollution levels
Research Article
soil
Soil contamination
Soil pollution
Waste Water Technology
Water Management
Water Pollution Control
Flow cytometry
Environmental diagnosis
Ecotoxicology
Terrestrial isopods
Environmental bioindicators
Hepatopancreas
Biologic indicators
ISSN:1614-7499, 0944-1344, 1614-7499
Online Access:Get full text
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Summary:Several studies have reported the high bioindication capacity of Isopoda (Crustacea, Oniscidea), which is related to their important ability to accumulate contaminants, usefulness in soil ecotoxicology and bioindication activities. Any change in the isopod population, diversity and life cycle can indicate relevant pollution levels. The analysis of target tissues, such as the hepatopancreas, is another emerging approach (from a cytologic/histological level) to detect contaminant accumulation from different sources. In this study, tissue disaggregation procedures were optimised in the hepatopancreas, and flow cytometry (FC) was applied to detect cell viability and several cell functions. After disaggregation, two hepatopancreatic cell types, small (S) and big (B), were still recognisable: they differed in morphology and behaviour. The analyses were conducted for the first time on isopods from sites under different conditions of ecological disturbance through cytometric re-interpretation of ecological-environmental parameters. Significant differences in cell functional parameters were found, highlighting that isopod hepatopancreatic cells can be efficiently analysed by FC and represent standardisable, early biological indicators, tracing environmental-induced stress through cytologic/histologic analyses.
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Responsible Editor: Ludek Blaha
ISSN:1614-7499
0944-1344
1614-7499
DOI:10.1007/s11356-023-31375-x