Use of Matrigel in culture affects cell phenotype and gene expression in the first trimester trophoblast cell line HTR8/SVneo

There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture...

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Vydáno v:Placenta (Eastbourne) Ročník 33; číslo 7; s. 586 - 588
Hlavní autoři: Highet, A.R., Zhang, V.J., Heinemann, G.K., Roberts, C.T.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Kidlington Elsevier Ltd 01.07.2012
Elsevier
Témata:
Biological and medical sciences
Cell Differentiation - genetics
Cell Line
Collagen - administration & dosage
Collagen - pharmacology
Drug Combinations
Embryology: invertebrates and vertebrates. Teratology
Endothelial Cells - physiology
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression - drug effects
Gestational Age
Humans
Integrin alpha1 - genetics
Internal Medicine
Keratins - genetics
Laminin - administration & dosage
Laminin - pharmacology
Matrigel
Matrix Metalloproteinase 2 - genetics
Obstetrics and Gynecology
Phenotype
Plastics
Proteoglycans - administration & dosage
Proteoglycans - pharmacology
Trophoblast
Trophoblasts - metabolism
Gene expression
Trophoblast
Matrigel
Pregnancy
Trophoblaste
Cell culture
Vertebrata
Phenotype
Cell line
Mammalia
Fetal membrane
First trimester
In vitro
ISSN:0143-4004, 1532-3102, 1532-3102
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Shrnutí:There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promoted formation of “endothelial-like” tubes and reduced mRNA expression of matrix metalloproteinase 2 (MMP2), cytokeratin 7 (KRT7) and integrin alpha 1 (ITGA1), while increasing VE-cadherin (CDH5) expression consistent with a vascular phenotype. This process may constitute part of the endothelial cell mimicry exhibited by endovascular EVTs invading the maternal spiral arteries. HTR8/SVneo appears to be phenotypically polymorphic and adopt endovascular morphology on Matrigel.
Bibliografie:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0143-4004
1532-3102
1532-3102
DOI:10.1016/j.placenta.2012.04.003