Use of Matrigel in culture affects cell phenotype and gene expression in the first trimester trophoblast cell line HTR8/SVneo

There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture...

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Published in:	Placenta (Eastbourne) Vol. 33; no. 7; pp. 586 - 588
Main Authors:	Highet, A.R., Zhang, V.J., Heinemann, G.K., Roberts, C.T.
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 01.07.2012 Elsevier
Subjects:	Biological and medical sciences Cell Differentiation - genetics Cell Line Collagen - administration & dosage Collagen - pharmacology Drug Combinations Embryology: invertebrates and vertebrates. Teratology Endothelial Cells - physiology Fundamental and applied biological sciences. Psychology Gene expression Gene Expression - drug effects Gestational Age Humans Integrin alpha1 - genetics Internal Medicine Keratins - genetics Laminin - administration & dosage Laminin - pharmacology Matrigel Matrix Metalloproteinase 2 - genetics Obstetrics and Gynecology Phenotype Plastics Proteoglycans - administration & dosage Proteoglycans - pharmacology Trophoblast Trophoblasts - metabolism Gene expression Trophoblast Matrigel Pregnancy Trophoblaste Cell culture Vertebrata Phenotype Cell line Mammalia Fetal membrane First trimester In vitro
ISSN:	0143-4004, 1532-3102, 1532-3102
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Abstract	There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promoted formation of “endothelial-like” tubes and reduced mRNA expression of matrix metalloproteinase 2 (MMP2), cytokeratin 7 (KRT7) and integrin alpha 1 (ITGA1), while increasing VE-cadherin (CDH5) expression consistent with a vascular phenotype. This process may constitute part of the endothelial cell mimicry exhibited by endovascular EVTs invading the maternal spiral arteries. HTR8/SVneo appears to be phenotypically polymorphic and adopt endovascular morphology on Matrigel.
AbstractList	There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promoted formation of “endothelial-like” tubes and reduced mRNA expression of matrix metalloproteinase 2 (MMP2), cytokeratin 7 (KRT7) and integrin alpha 1 (ITGA1), while increasing VE-cadherin (CDH5) expression consistent with a vascular phenotype. This process may constitute part of the endothelial cell mimicry exhibited by endovascular EVTs invading the maternal spiral arteries. HTR8/SVneo appears to be phenotypically polymorphic and adopt endovascular morphology on Matrigel. There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promoted formation of "endothelial-like" tubes and reduced mRNA expression of matrix metalloproteinase 2 (MMP2), cytokeratin 7 (KRT7) and integrin alpha 1 (ITGA1), while increasing VE-cadherin (CDH5) expression consistent with a vascular phenotype. This process may constitute part of the endothelial cell mimicry exhibited by endovascular EVTs invading the maternal spiral arteries. HTR8/SVneo appears to be phenotypically polymorphic and adopt endovascular morphology on Matrigel.There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promoted formation of "endothelial-like" tubes and reduced mRNA expression of matrix metalloproteinase 2 (MMP2), cytokeratin 7 (KRT7) and integrin alpha 1 (ITGA1), while increasing VE-cadherin (CDH5) expression consistent with a vascular phenotype. This process may constitute part of the endothelial cell mimicry exhibited by endovascular EVTs invading the maternal spiral arteries. HTR8/SVneo appears to be phenotypically polymorphic and adopt endovascular morphology on Matrigel. AbstractThere is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promoted formation of “endothelial-like” tubes and reduced mRNA expression of matrix metalloproteinase 2 ( MMP2), cytokeratin 7 ( KRT7) and integrin alpha 1 ( ITGA1), while increasing VE-cadherin ( CDH5) expression consistent with a vascular phenotype. This process may constitute part of the endothelial cell mimicry exhibited by endovascular EVTs invading the maternal spiral arteries. HTR8/SVneo appears to be phenotypically polymorphic and adopt endovascular morphology on Matrigel.
Author	Roberts, C.T. Zhang, V.J. Highet, A.R. Heinemann, G.K.
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Keywords	Gene expression Trophoblast Matrigel Pregnancy Trophoblaste Cell culture Vertebrata Phenotype Cell line Mammalia Fetal membrane First trimester In vitro
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Snippet	There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of... AbstractThere is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects...
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SubjectTerms	Biological and medical sciences Cell Differentiation - genetics Cell Line Collagen - administration & dosage Collagen - pharmacology Drug Combinations Embryology: invertebrates and vertebrates. Teratology Endothelial Cells - physiology Fundamental and applied biological sciences. Psychology Gene expression Gene Expression - drug effects Gestational Age Humans Integrin alpha1 - genetics Internal Medicine Keratins - genetics Laminin - administration & dosage Laminin - pharmacology Matrigel Matrix Metalloproteinase 2 - genetics Obstetrics and Gynecology Phenotype Plastics Proteoglycans - administration & dosage Proteoglycans - pharmacology Trophoblast Trophoblasts - metabolism
Title	Use of Matrigel in culture affects cell phenotype and gene expression in the first trimester trophoblast cell line HTR8/SVneo
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