Adipose tissue-derived ECM hydrogels and their use as 3D culture scaffold

Adipose tissue has the therapeutic capacity in the form of a fat graft, for example, for treatment of irradiation-induced scars and difficult to heal dermal wounds. For large-scale clinical application, an off-the-shelf product is warranted. In recent years, ECM-derived hydrogels are postulated to h...

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Vydané v:Artificial cells, nanomedicine, and biotechnology Ročník 47; číslo 1; s. 1693 - 1701
Hlavní autori: Getova, Vasilena E., van Dongen, Joris A., Brouwer, Linda A., Harmsen, Martin C.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England Taylor & Francis 01.12.2019
Taylor & Francis Ltd
Taylor & Francis Group
Predmet:
Adipose tissue
adipose tissue-derived stromal cells (ASC)
Angiogenesis
Cell adhesion & migration
Cell culture
Cell death
Cell migration
Cell survival
Connective tissues
Cytotoxicity
Extracellular matrix
Extracellular matrix (ECM)
Fibroblasts
Growth factors
Homeostasis
hydrogel
Hydrogels
Radiation
Reconstructive surgery
Scars
Survival
Tissue culture
Toxicity
Wound healing
Extracellular matrix (ECM)
adipose tissue
hydrogel
adipose tissue-derived stromal cells (ASC)
ISSN:2169-1401, 2169-141X, 2169-141X
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Shrnutí:Adipose tissue has the therapeutic capacity in the form of a fat graft, for example, for treatment of irradiation-induced scars and difficult to heal dermal wounds. For large-scale clinical application, an off-the-shelf product is warranted. In recent years, ECM-derived hydrogels are postulated to harbour therapeutic capacity and might even replicate the beneficial effects of adipose tissue. In normal homeostasis, the natural ECM acts as a deposit of growth factors, that releases them over time. In the healing of lesions, this might promote cell accumulation and proliferation which in turn stimulates angiogenesis and repair. The decellularization of tissue and the generation of hydrogels may leave cytotoxic traces. Therefore, our research assessed the cytotoxic effect of human adipose tissue-derived ECM hydrogels on connective tissue cells i.e. fibroblasts. The results showed no cytotoxicity, meaning the hydrogels caused no cell death. Cell migration and survival were observed when cultured in ECM hydrogels and followed for 7 days. Cell survival in the hydrogel was confirmed with CFDA staining and also cells showed the ability to penetrate and migrate throughout the gel. We conclude that ECM hydrogels are promising to use as innovative therapy for wound healing.
Bibliografia:ObjectType-Article-1
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ISSN:2169-1401
2169-141X
2169-141X
DOI:10.1080/21691401.2019.1608215