Pectin-coated boron nitride nanotubes: In vitro cyto-/immune-compatibility on RAW 264.7 macrophages

Boron nitride nanotubes (BNNTs) represent a new opportunity for drug delivery and clinical therapy. The present work has the objective to investigate pectin-coated BNNTs (P-BNNTs) for their biocompatibility on macrophage cultures, since these cells are among the first components of the immune system...

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Vydané v:Biochimica et biophysica acta Ročník 1860; číslo 4; s. 775 - 784
Hlavní autori: Rocca, Antonella, Marino, Attilio, Del Turco, Serena, Cappello, Valentina, Parlanti, Paola, Pellegrino, Mario, Golberg, Dmitri, Mattoli, Virgilio, Ciofani, Gianni
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Netherlands Elsevier B.V 01.04.2016
Predmet:
adverse effects
Animals
apoptosis
biocompatibility
Boron Compounds - chemistry
boron nitride
Boron nitride nanotubes
Cell Line
Coated Materials, Biocompatible - chemistry
confocal microscopy
Cytocompatibility
cytokines
Cytokines - biosynthesis
Cytokines - immunology
drugs
Gene Expression Regulation - immunology
genes
Immune system
inflammation
Macrophages
Macrophages - cytology
Macrophages - immunology
Macrophages - metabolism
Materials Testing
Mice
nanotubes
Nanotubes - chemistry
necrosis
oxidative stress
Pectins - chemistry
therapeutics
toxicity
transmission electron microscopy
Cytocompatibility
Macrophages
Boron nitride nanotubes
Immune system
ISSN:0304-4165, 0006-3002, 1872-8006
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Shrnutí:Boron nitride nanotubes (BNNTs) represent a new opportunity for drug delivery and clinical therapy. The present work has the objective to investigate pectin-coated BNNTs (P-BNNTs) for their biocompatibility on macrophage cultures, since these cells are among the first components of the immune system to interact with administered nanoparticles. As first step, the potential toxicity of P-BNNTs is verified in terms of proliferation, oxidative stress induction and apoptosis/necrosis phenomena. Thereafter, the modulation of immune cell response following P-BNNT exposure is evaluated at gene and protein level, in particular focusing on cytokine release. Finally, P-BNNT internalization is assessed through transmission electron microscopy and confocal microscopy. The results proved that P-BNNTs are not toxic for macrophages up to 50μg/ml after 24h of incubation. The cytokine expression is not affected by P-BNNT administration both at gene and protein level. Moreover, P-BNNTs are internalized by macrophages without impairments of the cell structures. Collected data suggest that P-BNNTs cause neither adverse effects nor inflammation processes in macrophages. These findings represent the first and fundamental step in immune compatibility evaluation of BNNTs, mandatory before any further pre-clinical testing. [Display omitted] •Pectin-coated boron nitride nanotubes (BNNTs) have been prepared and tested on RAW 264.7 macrophages.•This study represents the first investigation of BNNT immune compatibility.•Collected data indicate the absence of acute toxicity and no adverse immune response.•The suitability of BNNTs for biomedical applications is confirmed.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/j.bbagen.2016.01.020