Chromatographic characterisation, under highly aqueous conditions, of a molecularly imprinted polymer binding the herbicide 2,4-dichlorophenoxyacetic acid

The affinity of a 2,4-dichlorophenoxyacetic acid (2,4-D) molecularly imprinted polymer (MIP), which was synthesised directly in an aqueous organic solvent, for its template (2,4-D) was studied and compared with the affinity exhibited by two other reference (control) polymers, NIPA and NIPB, for the...

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Bibliographic Details
Published in:Analytica chimica acta Vol. 591; no. 1; pp. 22 - 28
Main Authors: Legido-Quigley, C., Oxelbark, J., De Lorenzi, E., Zurutuza-Elorza, A., Cormack, P.A.G.
Format: Journal Article Conference Proceeding
Language:English
Published: Amsterdam Elsevier B.V 15.05.2007
Elsevier
Subjects:
2,4-Dichlorophenoxyacetic acid
2,4-Dichlorophenoxyacetic Acid - chemistry
Adsorption isotherm
Analytical chemistry
Applied sciences
Buffers
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Affinity
Exact sciences and technology
Frontal chromatography
Global environmental pollution
Herbicides - chemistry
Methacrylates - chemistry
Molecular imprinting
Other chromatographic methods
Pollution
Pyridines - chemistry
Frontal chromatography
Molecular imprinting
2,4-Dichlorophenoxyacetic acid
Adsorption isotherm
Water
Phosphates
2,4-D
Molecular structure
Pesticides
Polymer
Selectivity
Herbicide
Organic solvent
Sample preparation
Environment
Packed column
Methanol
ISSN:0003-2670, 1873-4324, 1873-4324
Online Access:Get full text
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Summary:The affinity of a 2,4-dichlorophenoxyacetic acid (2,4-D) molecularly imprinted polymer (MIP), which was synthesised directly in an aqueous organic solvent, for its template (2,4-D) was studied and compared with the affinity exhibited by two other reference (control) polymers, NIPA and NIPB, for the same analyte. Zonal chromatography was performed to establish the optimal selectivity, expressed as imprinting factor (IF), under chromatographic conditions more aqueous than those described so far in the literature. Frontal analysis (FA) was performed on columns packed with these polymers, using an optimized mobile phase composed of methanol/phosphate buffer (50/50, v/v), to extract adsorption isotherm data and retrieve binding parameters from the best isotherm model. Surprisingly, the template had comparable and strong affinity for both MIP ( K = 3.8 × 10 4 M −1) and NIPA ( K = 1.9 × 10 4 M −1), although there was a marked difference in the saturation capacities of selective and non-selective sites, as one would expect for an imprinted polymer. NIPB acts as a true control polymer in the sense that it has relatively low affinity for the template ( K = 8.0 × 10 2 M −1). This work provides the first frontal chromatographic characterization of such a polymer in a water-rich environment over a wide concentration range. The significance of this work stems from the fact that the chromatographic approach used is generic and can be applied readily to other analytes, but also because there is an increasing demand for well-characterised imprinted materials that function effectively in aqueous media and are thus well-suited for analytical science applications involving, for example, biofluids and environmental water samples.
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ISSN:0003-2670
1873-4324
1873-4324
DOI:10.1016/j.aca.2007.01.022