Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: a call for surveillance and intervention

is a multidrug-resistant bacterium capable of forming biofilms. This study aimed to assess resistance of clinical isolates from Libyan hospitals to antipseudomonal antibiotics, the prevalence of selected extended-spectrum β-lactamases and carbapenemase genes among these isolates, and the microorgani...

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Bibliographic Details
Published in:Libyan journal of medicine Vol. 19; no. 1; p. 2344320
Main Authors: Gadaime, Nasrin K., Haddadin, Randa N., Shehabi, Asem A., Omran, Intisar N.
Format: Journal Article
Language:English
Published: United States Taylor & Francis Ltd 31.12.2024
Taylor & Francis
Taylor & Francis Group
Subjects:
Alginates
Anti-Bacterial Agents - pharmacology
Antibiotics
Antimicrobial agents
antimicrobial resistance
Bacterial Proteins
beta-Lactamases - genetics
biofilm
Biofilms
carbapenemase
Drug Resistance, Bacterial
ESBL
Hospitals
Libya
Original
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Surveillance
ESBL
Pseudomonas aeruginosa
carbapenemase
antimicrobial resistance
Libya
biofilm
ISSN:1993-2820, 1819-6357, 1819-6357
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Summary:is a multidrug-resistant bacterium capable of forming biofilms. This study aimed to assess resistance of clinical isolates from Libyan hospitals to antipseudomonal antibiotics, the prevalence of selected extended-spectrum β-lactamases and carbapenemase genes among these isolates, and the microorganisms' capacity for alginate and biofilm production. Forty-five isolates were collected from four hospitals in Benghazi and Derna, Libya. Antimicrobial susceptibility was determined using agar disc diffusion. The presence of resistance genes ( ) was screened using PCR. Biofilm formation was quantified via the crystal violet assay, while alginate production was measured spectrophotometrically. Resistance to antipseudomonal antibiotics ranged from 48.9% to 75.6%. The most prevalent resistance gene was blaNDM (26.7%), followed by blaGES-1 (17.8%). Moreover, all isolates demonstrated varying degrees of biofilm-forming ability and alginate production. No statistically significant correlation was found between biofilm formation and alginate production. The dissemination of resistant genes in P. aeruginosa, particularly carbapenemases, is of great concern. This issue is compounded by the bacteria's biofilm-forming capability. Urgent intervention and continuous surveillance are imperative to prevent further deterioration and the catastrophic spread of resistance among these formidable bacteria.
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ISSN:1993-2820
1819-6357
1819-6357
DOI:10.1080/19932820.2024.2344320