Dynamic repositioning of genes in the nucleus of lymphocytes preparing for cell division

We show that several transcriptionally inactive genes localize to centromeric heterochromatin in the nucleus of cycling but not quiescent (noncycling) primary B lymphocytes. In quiescent cells, centromeric repositioning of inactive loci was induced after mitogenic stimulation. A dynamic repositionin...

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Bibliographic Details
Published in:Molecular cell Vol. 3; no. 2; p. 207
Main Authors: Brown, K E, Baxter, J, Graf, D, Merkenschlager, M, Fisher, A G
Format: Journal Article
Language:English
Published: United States 01.02.1999
Subjects:
Alleles
Animals
B-Lymphocytes - cytology
Cell Division
Cell Nucleus - ultrastructure
Centromere - ultrastructure
DNA Nucleotidylexotransferase - genetics
DNA Nucleotidylexotransferase - metabolism
DNA-Binding Proteins
Gene Expression Regulation - physiology
Heterochromatin - genetics
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Ikaros Transcription Factor
In Situ Hybridization, Fluorescence
Mice
Microscopy, Confocal
T-Lymphocytes - cytology
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
ISSN:1097-2765
Online Access:Get more information
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Summary:We show that several transcriptionally inactive genes localize to centromeric heterochromatin in the nucleus of cycling but not quiescent (noncycling) primary B lymphocytes. In quiescent cells, centromeric repositioning of inactive loci was induced after mitogenic stimulation. A dynamic repositioning of selected genes was also observed in developing T cells. Rag and TdT loci were shown to relocate to centromeric domains following heritable gene silencing in primary CD4+8+ thymocytes, but not in a phenotypically similar cell line in which silencing occurred but was not heritable. Collectively, these data indicate that the spatial organization of genes in cycling and noncycling lymphocytes is different and that locus repositioning may be a feature of heritable gene silencing.
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ISSN:1097-2765
DOI:10.1016/s1097-2765(00)80311-1