Vitamin D improves endothelial dysfunction and restores myeloid angiogenic cell function via reduced CXCL-10 expression in systemic lupus erythematosus

Patients with systemic lupus erythematosus (SLE) have accelerated cardiovascular disease and dysfunctional endothelial repair mechanisms. Myeloid angiogenic cells (MACs), derived from circulating monocytes, augment vascular repair by paracrine secretion of pro-angiogenic factors. We observed that SL...

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Veröffentlicht in:Scientific reports Jg. 6; H. 1; S. 22341
Hauptverfasser: Reynolds, John A., Haque, Sahena, Williamson, Kate, Ray, David W., Alexander, M. Yvonne, Bruce, Ian N.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 01.03.2016
Nature Publishing Group
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Adult
Autoimmune diseases
Calcitriol - pharmacology
Cardiovascular diseases
Cell Adhesion - drug effects
Cell Count
Cell Differentiation - drug effects
Cell Movement - drug effects
Chemokine CXCL10 - metabolism
Down-Regulation - drug effects
Endothelium, Vascular - drug effects
Endothelium, Vascular - physiopathology
Health risks
Humanities and Social Sciences
Humans
Immunology
Interferons - metabolism
Lupus
Lupus Erythematosus, Systemic - metabolism
Lupus Erythematosus, Systemic - physiopathology
Middle Aged
Models, Biological
multidisciplinary
Myeloid Cells - drug effects
Myeloid Cells - pathology
Neovascularization, Physiologic - drug effects
Nitric Oxide Synthase Type III - metabolism
Phenotype
Science
Systemic lupus erythematosus
Vitamin D
Vitamin D - pharmacology
ISSN:2045-2322, 2045-2322
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Zusammenfassung:Patients with systemic lupus erythematosus (SLE) have accelerated cardiovascular disease and dysfunctional endothelial repair mechanisms. Myeloid angiogenic cells (MACs), derived from circulating monocytes, augment vascular repair by paracrine secretion of pro-angiogenic factors. We observed that SLE MACs are dysfunctional and secrete pro-inflammatory cytokines. We also found that the vitamin D receptor was transiently expressed during MAC differentiation and that in vitro , calcitriol increased differentiation of monocytes into MACs in both SLE and in a model using the prototypic SLE cytokine, interferon-alpha. The active form of vitamin D (calcitriol) restored the SLE MAC phenotype towards that of healthy subjects with reduced IL-6 secretion and normalised surface marker expression. Calcitriol also augmented the angiogenic capacity of MACs via the down-regulation of CXCL-10. In SLE patients treated with cholecalciferol for 12 weeks, the improvement in endothelial function correlated with increase in serum 25(OH)D concentrations independently of disease activity. We also show that MACs were able to positively modulate eNOS expression in human endothelial cells in vitro, an effect further enhanced by calcitriol treatment of SLE MACs. The results demonstrate that vitamin D can positively modify endothelial repair mechanisms and thus endothelial function in a population with significant cardiovascular risk.
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ISSN:2045-2322
2045-2322
DOI:10.1038/srep22341