Combination of serological total antibody and RT-PCR test for detection of SARS-COV-2 infections

•Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections.•Wantai total antibody test by Chemiluminescence Microparticle Immunoassay (CMIA) against SARS-COV-2 Infections.•Analysis of false positive of false negative results by Wantai total antibody test. The...

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Published in:	Journal of virological methods Vol. 283; p. 113919
Main Author:	Wang, Pei
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01.09.2020
Subjects:	Adolescent Adult Aged antibodies Antibodies, Viral - blood Antibodies, Viral - immunology Betacoronavirus - immunology blood serum Case-Control Studies Chemiluminescence microparticle immunoassay China Coronavirus Infections - diagnosis Coronavirus Infections - immunology COVID-19 COVID-19 infection diagnostic sensitivity epidemiological studies Female Humans Immunoassay - methods Luminescent Measurements - methods Male Middle Aged Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - immunology Real -Time RT-PCR Real-Time Polymerase Chain Reaction - methods Retrospective Studies reverse transcriptase polymerase chain reaction SARS-COV-2 Sensitivity and Specificity Serologic Tests - methods Serology Severe acute respiratory syndrome coronavirus 2 throat Young Adult China COVID-19 SARS-COV-2 RT-PCR Chemiluminescence microparticle immunoassay RLU Serology CMIA Real -Time RT-PCR
ISSN:	0166-0934, 1879-0984, 1879-0984
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Abstract	•Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections.•Wantai total antibody test by Chemiluminescence Microparticle Immunoassay (CMIA) against SARS-COV-2 Infections.•Analysis of false positive of false negative results by Wantai total antibody test. The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X2 = 5.16, P < 0.05), and similar to that of total antibody tests (X2 = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2.
AbstractList	The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X2 = 5.16, P < 0.05), and similar to that of total antibody tests (X2 = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2.The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X2 = 5.16, P < 0.05), and similar to that of total antibody tests (X2 = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2. • Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections. • Wantai total antibody test by Chemiluminescence Microparticle Immunoassay (CMIA) against SARS-COV-2 Infections. • Analysis of false positive of false negative results by Wantai total antibody test. The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X2 = 5.16, P < 0.05), and similar to that of total antibody tests (X2 = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2. The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X = 5.16, P < 0.05), and similar to that of total antibody tests (X = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2. •Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections.•Wantai total antibody test by Chemiluminescence Microparticle Immunoassay (CMIA) against SARS-COV-2 Infections.•Analysis of false positive of false negative results by Wantai total antibody test. The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X2 = 5.16, P < 0.05), and similar to that of total antibody tests (X2 = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2. The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X² = 5.16, P < 0.05), and similar to that of total antibody tests (X² = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2.
ArticleNumber	113919
Author	Wang, Pei
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Snippet	•Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections.•Wantai total antibody test by Chemiluminescence... The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted... • Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections. • Wantai total antibody test by Chemiluminescence...
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SubjectTerms	Adolescent Adult Aged antibodies Antibodies, Viral - blood Antibodies, Viral - immunology Betacoronavirus - immunology blood serum Case-Control Studies Chemiluminescence microparticle immunoassay China Coronavirus Infections - diagnosis Coronavirus Infections - immunology COVID-19 COVID-19 infection diagnostic sensitivity epidemiological studies Female Humans Immunoassay - methods Luminescent Measurements - methods Male Middle Aged Pandemics Pneumonia, Viral - diagnosis Pneumonia, Viral - immunology Real -Time RT-PCR Real-Time Polymerase Chain Reaction - methods Retrospective Studies reverse transcriptase polymerase chain reaction SARS-COV-2 Sensitivity and Specificity Serologic Tests - methods Serology Severe acute respiratory syndrome coronavirus 2 throat Young Adult
Title	Combination of serological total antibody and RT-PCR test for detection of SARS-COV-2 infections
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