Combination of serological total antibody and RT-PCR test for detection of SARS-COV-2 infections

•Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections.•Wantai total antibody test by Chemiluminescence Microparticle Immunoassay (CMIA) against SARS-COV-2 Infections.•Analysis of false positive of false negative results by Wantai total antibody test. The...

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Bibliographic Details
Published in:Journal of virological methods Vol. 283; p. 113919
Main Author: Wang, Pei
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01.09.2020
Subjects:
Adolescent
Adult
Aged
antibodies
Antibodies, Viral - blood
Antibodies, Viral - immunology
Betacoronavirus - immunology
blood serum
Case-Control Studies
Chemiluminescence microparticle immunoassay
China
Coronavirus Infections - diagnosis
Coronavirus Infections - immunology
COVID-19
COVID-19 infection
diagnostic sensitivity
epidemiological studies
Female
Humans
Immunoassay - methods
Luminescent Measurements - methods
Male
Middle Aged
Pandemics
Pneumonia, Viral - diagnosis
Pneumonia, Viral - immunology
Real -Time RT-PCR
Real-Time Polymerase Chain Reaction - methods
Retrospective Studies
reverse transcriptase polymerase chain reaction
SARS-COV-2
Sensitivity and Specificity
Serologic Tests - methods
Serology
Severe acute respiratory syndrome coronavirus 2
throat
Young Adult
China
COVID-19
SARS-COV-2
RT-PCR
Chemiluminescence microparticle immunoassay
RLU
Serology
CMIA
Real -Time RT-PCR
ISSN:0166-0934, 1879-0984, 1879-0984
Online Access:Get full text
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Summary:•Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections.•Wantai total antibody test by Chemiluminescence Microparticle Immunoassay (CMIA) against SARS-COV-2 Infections.•Analysis of false positive of false negative results by Wantai total antibody test. The purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-COV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-COV-2 from 25th January to 16th March 2020. Patients were divided into a COVID-19 group (n = 141) and a control group (n = 234). Serum samples and throat swabs were collected from 375 patients for total antibody testing against SARS-COV-2 and RT-PCR analysis, respectively. The results indicated that diagnostic sensitivity and specificity were 95.7 % and 98.7 %, 92.2 % and 100 % by total antibody tests and RT-PCR, respectively. The sensitivity and specificity of total antibody tests combined with RT-PCR were 98.6 % and 98.7 %. The sensitivity of the combined method was significantly higher than RT-PCR (X2 = 5.16, P < 0.05), and similar to that of total antibody tests (X2 = 1.15, P> 0.05). This study supported the advantage of the combined method for detection of SARS-COV-2 with a high degree of sensitivity and specificity, as a useful tool for accurate diagnosis and timely treatment of suspected patients, epidemiological investigation, as well as monitoring ongoing outbreaks of infections with SARS-COV-2.
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ISSN:0166-0934
1879-0984
1879-0984
DOI:10.1016/j.jviromet.2020.113919