Distinct signatures of dental plaque metabolic byproducts dictated by periodontal inflammatory status

Onset of chronic periodontitis is associated with an aberrant polymicrobial community, termed dysbiosis. Findings regarding its etiology obtained using high-throughput sequencing technique suggested that dysbiosis holds a conserved metabolic signature as an emergent property. The purpose of this stu...

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Vydané v:Scientific reports Ročník 7; číslo 1; s. 42818
Hlavní autori: Sakanaka, Akito, Kuboniwa, Masae, Hashino, Ei, Bamba, Takeshi, Fukusaki, Eiichiro, Amano, Atsuo
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 21.02.2017
Nature Publishing Group
Predmet:
631/45/320
692/53/2421
692/699/255/1318
Adult
Area Under Curve
Arginine
Biomarkers
Biomarkers - metabolism
Butyric acid
Cadaverine
Cadaverine - metabolism
Chronic Periodontitis - metabolism
Chronic Periodontitis - pathology
Dental plaque
Dental Plaque - metabolism
Dysbacteriosis
Ethanolamine
Etiology
Female
Humanities and Social Sciences
Humans
Inflammation
Lysine
Male
Metabolism
Metabolites
Metabolomics
Middle Aged
multidisciplinary
Next-generation sequencing
Periodontitis
Phenylpropionates - metabolism
Proline
ROC Curve
Saliva
Saliva - metabolism
Science
Severity of Illness Index
Uric acid
ISSN:2045-2322, 2045-2322
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Shrnutí:Onset of chronic periodontitis is associated with an aberrant polymicrobial community, termed dysbiosis. Findings regarding its etiology obtained using high-throughput sequencing technique suggested that dysbiosis holds a conserved metabolic signature as an emergent property. The purpose of this study was to identify robust biomarkers for periodontal inflammation severity. Furthermore, we investigated disease-associated metabolic signatures of periodontal microbiota using a salivary metabolomics approach. Whole saliva samples were obtained from adult subjects before and after removal of supragingival plaque (debridement). Periodontal inflamed surface area (PISA) was employed as an indicator of periodontal inflammatory status. Based on multivariate analyses using pre-debridement salivary metabolomics data, we found that metabolites associated with higher PISA included cadaverine and hydrocinnamate, while uric acid and ethanolamine were associated with lower PISA. Next, we focused on dental plaque metabolic byproducts by selecting salivary metabolites significantly decreased following debridement. Metabolite set enrichment analysis revealed that polyamine metabolism, arginine and proline metabolism, butyric acid metabolism, and lysine degradation were distinctive metabolic signatures of dental plaque in the high PISA group, which may be related to the metabolic signatures of disease-associated communities. Collectively, our findings identified potential biomarkers of periodontal inflammatory status and also provide insight into metabolic signatures of dysbiotic communities.
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ISSN:2045-2322
2045-2322
DOI:10.1038/srep42818