LncRNA—UCA1 enhances cell proliferation and 5-fluorouracil resistance in colorectal cancer by inhibiting miR-204-5p

Recent preliminary studies reported the in vitro tumor-promoting effects of long non-coding RNA urothelial carcinoma associated 1 (UCA1) in colorectal cancer (CRC). However, the in vivo functions and molecular mechanism of UCA1 in CRC remain unclear. Therefore, we investigated the detailed role and...

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Bibliographic Details
Published in:Scientific reports Vol. 6; no. 1; p. 23892
Main Authors: Bian, Zehua, Jin, Liugen, Zhang, Jiwei, Yin, Yuan, Quan, Chao, Hu, Yaling, Feng, Yuyang, Liu, Heyong, Fei, Bojian, Mao, Yong, Zhou, Leyuan, Qi, Xiaowei, Huang, Shenlin, Hua, Dong, Xing, Chungen, Huang, Zhaohui
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 05.04.2016
Nature Publishing Group
Subjects:
13/105
13/109
13/2
13/44
13/51
13/89
38/77
5-Fluorouracil
631/208/200
631/67/1504/1885
Animals
Apoptosis
Bladder cancer
Cell Line, Tumor
Cell Proliferation
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - drug therapy
Colorectal Neoplasms - genetics
Cyclic AMP Response Element-Binding Protein - genetics
Cyclic AMP Response Element-Binding Protein - metabolism
Drug Resistance, Neoplasm - genetics
Fluorouracil - chemistry
Gene Expression Regulation, Neoplastic
Genetic Vectors
HEK293 Cells
Humanities and Social Sciences
Humans
Male
Medical prognosis
Mice
Mice, Nude
MicroRNAs - genetics
multidisciplinary
Non-coding RNA
Oncogenes
Prognosis
RNA, Long Noncoding - genetics
Science
Survival
Transcriptional Activation
Up-Regulation
Urothelial carcinoma
ISSN:2045-2322, 2045-2322
Online Access:Get full text
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Summary:Recent preliminary studies reported the in vitro tumor-promoting effects of long non-coding RNA urothelial carcinoma associated 1 (UCA1) in colorectal cancer (CRC). However, the in vivo functions and molecular mechanism of UCA1 in CRC remain unclear. Therefore, we investigated the detailed role and mechanism of UCA1 in CRC. We found that UCA1 was up-regulated in CRCs and negatively correlated with survival time in two CRC cohorts. Functional assays revealed the in vitro and in vivo growth-promoting function of UCA1 and revealed that UCA1 can decrease the sensitivity of CRC cells to 5-FU by attenuating apoptosis. Further mechanistic studies revealed that UCA1 could sponge endogenous miR-204-5p and inhibit its activity. We also identified CREB1 as a new target of miR-204-5p. The protein levels of CREB1 were significantly up-regulated in CRCs, negatively associated with survival time and positively correlated with the UCA1 expression. The present work provides the first evidence of a UCA1-miR-204-5p- CREB1 / BCL2 / RAB22A regulatory network in CRC and reveals that UCA1 and CREB1 are potential new oncogenes and prognostic factors for CRC.
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ISSN:2045-2322
2045-2322
DOI:10.1038/srep23892