Total Extracellular Small RNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects

Interest in circulating RNAs for monitoring and diagnosing human health has grown significantly. There are few datasets describing baseline expression levels for total cell-free circulating RNA from healthy control subjects. In this study, total extracellular RNA (exRNA) was isolated and sequenced f...

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Vydané v:Scientific reports Ročník 7; číslo 1; s. 44061
Hlavní autori: Yeri, Ashish, Courtright, Amanda, Reiman, Rebecca, Carlson, Elizabeth, Beecroft, Taylor, Janss, Alex, Siniard, Ashley, Richholt, Ryan, Balak, Chris, Rozowsky, Joel, Kitchen, Robert, Hutchins, Elizabeth, Winarta, Joseph, McCoy, Roger, Anastasi, Matthew, Kim, Seungchan, Huentelman, Matthew, Van Keuren-Jensen, Kendall
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 17.03.2017
Nature Publishing Group
Predmet:
631/208/199
631/208/514/1949
Adolescent
Adult
Athletes
Cell-Free Nucleic Acids - analysis
Cell-Free Nucleic Acids - isolation & purification
Humanities and Social Sciences
Humans
Male
miRNA
multidisciplinary
Ribonucleic acid
RNA
RNA - analysis
RNA - blood
RNA - isolation & purification
RNA - urine
Saliva
Saliva - chemistry
Science
Student athletes
tRNA
Urine
Young Adult
ISSN:2045-2322, 2045-2322
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Shrnutí:Interest in circulating RNAs for monitoring and diagnosing human health has grown significantly. There are few datasets describing baseline expression levels for total cell-free circulating RNA from healthy control subjects. In this study, total extracellular RNA (exRNA) was isolated and sequenced from 183 plasma samples, 204 urine samples and 46 saliva samples from 55 male college athletes ages 18–25 years. Many participants provided more than one sample, allowing us to investigate variability in an individual’s exRNA expression levels over time. Here we provide a systematic analysis of small exRNAs present in each biofluid, as well as an analysis of exogenous RNAs. The small RNA profile of each biofluid is distinct. We find that a large number of RNA fragments in plasma (63%) and urine (54%) have sequences that are assigned to YRNA and tRNA fragments respectively. Surprisingly, while many miRNAs can be detected, there are few miRNAs that are consistently detected in all samples from a single biofluid, and profiles of miRNA are different for each biofluid. Not unexpectedly, saliva samples have high levels of exogenous sequence that can be traced to bacteria. These data significantly contribute to the current number of sequenced exRNA samples from normal healthy individuals.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:2045-2322
2045-2322
DOI:10.1038/srep44061