COVID-19: S-Peptide RBD 484–508 Induces IFN-γ T-Cell Response in Naïve-to-Infection and Unvaccinated Subjects with Close Contact with SARS-CoV-2-Positive Patients

Despite the availability on the market of different anti-SARS-CoV-2 vaccines, there are still unanswered questions on whether they can stimulate long-lasting protection. A deep understanding of adaptive immune response to SARS-CoV-2 is important for optimizing both vaccine development and pandemic c...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Viruses Jg. 15; H. 7; S. 1417
Hauptverfasser: Murdocca, Michela, Citro, Gennaro, Centanini, Eleonora, Giannini, Rosalinda, Latini, Andrea, Centofanti, Federica, Piano Mortari, Eva, Cocciadiferro, Dario, Novelli, Antonio, Bernardini, Sergio, Novelli, Giuseppe, Sangiuolo, Federica
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland MDPI AG 22.06.2023
MDPI
Schlagworte:
Adaptive immunity
Antibodies
Autoantibodies
Blood
Cells
Communication
COVID-19
COVID-19 infection
Cytokines
Gene expression
Genetic diversity
Genomes
IFN-γ
Immune response
Infections
interferons
Lymphocytes
Lymphocytes T
Manufacturers
markets
pandemic
peptide
Peptides
protein synthesis
Pseudovirus
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Spike protein
T-lymphocytes
Vaccine development
Vaccines
γ-Interferon
St Louis Missouri
United States > US
pseudovirus
IFN-γ
SARS-CoV-2
peptide
ISSN:1999-4915, 1999-4915
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Despite the availability on the market of different anti-SARS-CoV-2 vaccines, there are still unanswered questions on whether they can stimulate long-lasting protection. A deep understanding of adaptive immune response to SARS-CoV-2 is important for optimizing both vaccine development and pandemic control measures. Among cytokines secreted by lymphocytes in response to viral infection, IFN-γ plays a pivotal role both in innate and adaptive immunity. In this study, we report on 28 naïve-to-SARS-Cov-2-infection and unvaccinated subjects, having reported a close and prolonged contact with COVID-19-positive patients. Samples were tested for defective genetic variants in interferon pathway genes by whole exome sequencing and anti-IFN autoantibodies production was investigated. Subject T-cells were cultured and infected with pseudotype particles bearing the S proteins and in parallel stimulated with two S-peptides designed on the RBD region of the spike protein. Our results showed that one of these peptides, RBD 484–508, induces a significant increase in IFN-γ gene expression and protein production in T-cells, comparable to those obtained in cells infected by SARS-CoV-2 pseudovirus. This work deepens our understanding of immune response and highlights the selected peptide as a reasonable approach to induce broad, potent, and variant concern-independent T-cell responses.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1999-4915
1999-4915
DOI:10.3390/v15071417