Development of a Pan-Filoviridae SYBR Green qPCR Assay for Biosurveillance Studies in Bats

Recent studies have indicated that bats are hosts to diverse filoviruses. Currently, no pan-filovirus molecular assays are available that have been evaluated for the detection of all mammalian filoviruses. In this study, a two-step pan-filovirus SYBR Green real-time PCR assay targeting the nucleopro...

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Bibliographic Details
Published in:Viruses Vol. 15; no. 4; p. 987
Main Authors: Coertse, Jessica, Mortlock, Marinda, Grobbelaar, Antoinette, Moolla, Naazneen, Markotter, Wanda, Weyer, Jacqueline
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 17.04.2023
MDPI
Subjects:
Animals
Bats
Biosurveillance
Chiroptera
detection limit
Ebolavirus - genetics
Epidemics
Fever
Filoviridae
Filoviridae - genetics
genes
Hemorrhagic Fever, Ebola
Infectious diseases
Mammals
Marburgvirus
monitoring
nucleoproteins
pan-filovirus
Pathogens
qPCR
quantitative polymerase chain reaction
RNA polymerase
Surveillance
SYBR Green
Viruses
Zoonoses
Sierra Leone
United States > US
Germany
Sudan
bats
qPCR
surveillance
pan-filovirus
SYBR Green
ISSN:1999-4915, 1999-4915
Online Access:Get full text
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Summary:Recent studies have indicated that bats are hosts to diverse filoviruses. Currently, no pan-filovirus molecular assays are available that have been evaluated for the detection of all mammalian filoviruses. In this study, a two-step pan-filovirus SYBR Green real-time PCR assay targeting the nucleoprotein gene was developed for filovirus surveillance in bats. Synthetic constructs were designed as representatives of nine filovirus species and used to evaluate the assay. This assay detected all synthetic constructs included with an analytical sensitivity of 3–31.7 copies/reaction and was evaluated against the field collected samples. The assay’s performance was similar to a previously published probe based assay for detecting Ebola- and Marburgvirus. The developed pan-filovirus SYBR Green assay will allow for more affordable and sensitive detection of mammalian filoviruses in bat samples.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v15040987