Synergistic virulence of Porphyromonas gingivalis and Treponema denticola in a murine periodontitis model

Summary Chronic periodontitis is characterized by the destruction of the tissues supporting the teeth and has been associated with the presence of a subgingival polymicrobial biofilm containing Porphyromonas gingivalis and Treponema denticola. We have investigated the potential synergistic virulence...

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Vydáno v:	Molecular oral microbiology Ročník 26; číslo 4; s. 229 - 240
Hlavní autoři:	Orth, R.K-H., O'Brien-Simpson, N.M., Dashper, S.G., Reynolds, E.C.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Oxford, UK Blackwell Publishing Ltd 01.08.2011
Témata:	alveolar bone loss Alveolar Bone Loss - microbiology animal model Animal models Animals Biofilms Bone (alveolar) Bone loss chronic periodontitis Chronic Periodontitis - immunology Chronic Periodontitis - microbiology Cytokines Data processing Disease Models, Animal DNA, Bacterial - analysis gamma -Interferon Immune response Inoculation Interferon-gamma - biosynthesis Interleukin-4 - biosynthesis Leukocyte Count Lymphocytes T Mice Mice, Inbred BALB C Microbial Interactions - physiology Periodontitis Porphyromonas Porphyromonas gingivalis Porphyromonas gingivalis - immunology T-Lymphocytes - immunology Teeth Treponema Treponema denticola Treponema denticola - immunology Virulence
ISSN:	2041-1006, 2041-1014, 2041-1014
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Shrnutí:	Summary Chronic periodontitis is characterized by the destruction of the tissues supporting the teeth and has been associated with the presence of a subgingival polymicrobial biofilm containing Porphyromonas gingivalis and Treponema denticola. We have investigated the potential synergistic virulence of P. gingivalis and T. denticola using a murine experimental model of periodontitis. An inoculation regime of four intra‐oral doses of 1 × 1010P. gingivalis cells induced significant periodontal bone loss compared with loss in sham‐inoculated mice, whereas doses of 1 × 109 cells or lower did not induce bone loss. Inoculation with T. denticola with up to eight doses of 1 × 1010 cells failed to induce bone loss in this model. However, four doses of a co‐inoculum of a 1 : 1 ratio of P. gingivalis and T. denticola at 5 × 108 or 1 × 109 total bacterial cells induced the same level of bone loss as four doses of 1 × 1010P. gingivalis cells. Co‐inoculation induced strong P. gingivalis‐specific T‐cell proliferative and interferon‐γ‐dominant cytokine responses, and induced a strong T. denticola‐specific interferon‐γ dominant cytokine response. Only at the higher co‐inoculum dose of 1 × 1010 total cells was a T. denticola‐specific T‐cell proliferative response observed. These data show that P. gingivalis and T. denticola act synergistically to stimulate the host immune response and to induce alveolar bone loss in a murine experimental periodontitis model.
Bibliografie:	istex:21AE5D19DFAA7661FC6C87CFBA99D20965A0B95A ark:/67375/WNG-8L3JPW89-X ArticleID:MOM612 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	2041-1006 2041-1014 2041-1014
DOI:	10.1111/j.2041-1014.2011.00612.x