Systematic genome-wide screens of gene function

Key Points High-throughput screens, in which each gene of an organism is systematically perturbed, are now routine in yeast and are becoming feasible in other organisms. Before conducting a genome-wide screen, the list of gene sequences to be targeted must be compiled and a set of gene-perturbing re...

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Veröffentlicht in:	Nature reviews. Genetics Jg. 5; H. 1; S. 11 - 22
Hauptverfasser:	Carpenter, Anne E., Sabatini, David M.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London Nature Publishing Group UK 01.01.2004 Nature Publishing Group
Schlagworte:	Agriculture Animal Genetics and Genomics Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Caenorhabditis elegans Caenorhabditis elegans - genetics Cancer Research Diverse techniques Drosophila melanogaster Drosophila melanogaster - genetics Fundamental and applied biological sciences. Psychology Gene Function Genes Genes, Reporter Genetic Techniques Genome Genome, Fungal Genome, Human Genomes Genomics Human Genetics Humans Insects Molecular and cellular biology Mutagenesis Mutation Nematodes Organisms Phenotype Proteins Reagents Recombination, Genetic review-article RNA Interference Saccharomyces cerevisiae - genetics Yeast Caenorhabditis elegans Screening Animal model Targeting Mutagenesis Helmintha Nemathelminthia Method Invertebrata Genome Nematoda Functional genomics
ISSN:	1471-0056, 1471-0064
Online-Zugang:	Volltext
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Zusammenfassung:	Key Points High-throughput screens, in which each gene of an organism is systematically perturbed, are now routine in yeast and are becoming feasible in other organisms. Before conducting a genome-wide screen, the list of gene sequences to be targeted must be compiled and a set of gene-perturbing reagents or strains must be constructed. Gene-perturbing strategies include: homologous recombination and random insertional mutagenesis to delete or mutate genes at the DNA level, and RNA interference to reduce the mRNA levels of a gene. Any observable phenotype can be screened using genome-wide collections of reagents or organisms, subject to practical limitations. Examples of phenotypes that have been screened so far include cell growth and proliferation, classical morphological defects and reporter-gene activity. Using automated microscopy and/or automated image analysis, visual phenotypes can also be screened in a high-throughput manner. Genome-wide phenotypic screens generate large, high-quality data sets, which can be used immediately to identify genes that are involved in a particular process. These genome-wide data sets can also be integrated with existing genome-wide data sets, such as transcriptional profiles and annotated databases, to provide further, broader insights. Future screens will probably examine more complex phenotypes in increasingly physiological contexts. By using genome information to create tools for perturbing gene function, it is now possible to undertake systematic genome-wide functional screens that examine the contribution of every gene to a biological process. The directed nature of these experiments contrasts with traditional methods, in which random mutations are induced and the resulting mutants are screened for various phenotypes. The first genome-wide functional screens in Caenorhabditis elegans and Drosophila melanogaster have recently been published, and screens in human cells will soon follow. These high-throughput techniques promise the rapid annotation of genomes with high-quality information about the biological function of each gene.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 ObjectType-Review-3 content type line 23
ISSN:	1471-0056 1471-0064
DOI:	10.1038/nrg1248