Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study

Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed...

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Bibliographic Details
Published in:Anesthesia and analgesia Vol. 129; no. 5; p. e150
Main Authors: Berger, Miles, Murdoch, David M, Staats, Janet S, Chan, Cliburn, Thomas, Jake P, Garrigues, Grant E, Browndyke, Jeffrey N, Cooter, Mary, Quinones, Quintin J, Mathew, Joseph P, Weinhold, Kent J
Format: Journal Article
Language:English
Published: United States 01.11.2019
Subjects:
Cerebrospinal Fluid - cytology
Flow Cytometry - methods
GPI-Linked Proteins - analysis
Humans
Lipopolysaccharide Receptors - analysis
Monocytes - immunology
Pilot Projects
Postoperative Cognitive Complications - cerebrospinal fluid
Postoperative Cognitive Complications - etiology
Receptors, IgG - analysis
ISSN:1526-7598, 1526-7598
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Summary:Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.
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ISSN:1526-7598
1526-7598
DOI:10.1213/ANE.0000000000004179