Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study

Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed...

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Vydáno v:	Anesthesia and analgesia Ročník 129; číslo 5; s. e150
Hlavní autoři:	Berger, Miles, Murdoch, David M, Staats, Janet S, Chan, Cliburn, Thomas, Jake P, Garrigues, Grant E, Browndyke, Jeffrey N, Cooter, Mary, Quinones, Quintin J, Mathew, Joseph P, Weinhold, Kent J
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 01.11.2019
Témata:	Cerebrospinal Fluid - cytology Flow Cytometry - methods GPI-Linked Proteins - analysis Humans Lipopolysaccharide Receptors - analysis Monocytes - immunology Pilot Projects Postoperative Cognitive Complications - cerebrospinal fluid Postoperative Cognitive Complications - etiology Receptors, IgG - analysis
ISSN:	1526-7598, 1526-7598
On-line přístup:	Zjistit podrobnosti o přístupu
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Shrnutí:	Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1526-7598 1526-7598
DOI:	10.1213/ANE.0000000000004179