Fibrillin-1 and elastin are differentially expressed in hypertrophic scars and keloids

Hypertrophic scars and keloids are two forms of excessive cutaneous scarring. Considering the importance of extracellular matrix elements in tissue repair, a morphological and quantitative analysis of the elastic system components (fibrillin‐1 and elastin) was performed in normal skin, normal scars,...

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Published in:Wound repair and regeneration Vol. 12; no. 2; pp. 169 - 174
Main Authors: Amadeu, Thaís P., Braune, André S., Porto, Luís C., Desmoulière, Alexis, Costa, Andréa M. A.
Format: Journal Article
Language:English
Published: Oxford, UK; Malden, USA Blackwell Science Inc 01.03.2004
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ISSN:1067-1927, 1524-475X
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Abstract Hypertrophic scars and keloids are two forms of excessive cutaneous scarring. Considering the importance of extracellular matrix elements in tissue repair, a morphological and quantitative analysis of the elastic system components (fibrillin‐1 and elastin) was performed in normal skin, normal scars, hypertrophic scars, and keloids. In superficial and deep dermis, fibrillin‐1 volume density was significantly higher in normal skin compared with normal scars, hypertrophic scars, and keloids. The fibrillin‐1 volume density did not show differences between hypertrophic scars and keloids in superficial or deep dermis. In superficial dermis, elastin volume density was higher in normal skin compared with normal scars, hypertrophic scars, and keloids. In deep dermis, the elastin volume density was higher in keloids compared with normal skins, normal scars, and hypertrophic scars. We showed that the distribution of fibrillin‐1 and elastin is disrupted in all kinds of scars analyzed, but there are two patterns: one for normal scars and another for excessive scars.
AbstractList Hypertrophic scars and keloids are two forms of excessive cutaneous scarring. Considering the importance of extracellular matrix elements in tissue repair, a morphological and quantitative analysis of the elastic system components (fibrillin-1 and elastin) was performed in normal skin, normal scars, hypertrophic scars, and keloids. In superficial and deep dermis, fibrillin-1 volume density was significantly higher in normal skin compared with normal scars, hypertrophic scars, and keloids. The fibrillin-1 volume density did not show differences between hypertrophic scars and keloids in superficial or deep dermis. In superficial dermis, elastin volume density was higher in normal skin compared with normal scars, hypertrophic scars, and keloids. In deep dermis, the elastin volume density was higher in keloids compared with normal skins, normal scars, and hypertrophic scars. We showed that the distribution of fibrillin-1 and elastin is disrupted in all kinds of scars analyzed, but there are two patterns: one for normal scars and another for excessive scars.
Hypertrophic scars and keloids are two forms of excessive cutaneous scarring. Considering the importance of extracellular matrix elements in tissue repair, a morphological and quantitative analysis of the elastic system components (fibrillin-1 and elastin) was performed in normal skin, normal scars, hypertrophic scars, and keloids. In superficial and deep dermis, fibrillin-1 volume density was significantly higher in normal skin compared with normal scars, hypertrophic scars, and keloids. The fibrillin-1 volume density did not show differences between hypertrophic scars and keloids in superficial or deep dermis. In superficial dermis, elastin volume density was higher in normal skin compared with normal scars, hypertrophic scars, and keloids. In deep dermis, the elastin volume density was higher in keloids compared with normal skins, normal scars, and hypertrophic scars. We showed that the distribution of fibrillin-1 and elastin is disrupted in all kinds of scars analyzed, but there are two patterns: one for normal scars and another for excessive scars.Hypertrophic scars and keloids are two forms of excessive cutaneous scarring. Considering the importance of extracellular matrix elements in tissue repair, a morphological and quantitative analysis of the elastic system components (fibrillin-1 and elastin) was performed in normal skin, normal scars, hypertrophic scars, and keloids. In superficial and deep dermis, fibrillin-1 volume density was significantly higher in normal skin compared with normal scars, hypertrophic scars, and keloids. The fibrillin-1 volume density did not show differences between hypertrophic scars and keloids in superficial or deep dermis. In superficial dermis, elastin volume density was higher in normal skin compared with normal scars, hypertrophic scars, and keloids. In deep dermis, the elastin volume density was higher in keloids compared with normal skins, normal scars, and hypertrophic scars. We showed that the distribution of fibrillin-1 and elastin is disrupted in all kinds of scars analyzed, but there are two patterns: one for normal scars and another for excessive scars.
Author Costa, Andréa M. A.
Braune, André S.
Desmoulière, Alexis
Amadeu, Thaís P.
Porto, Luís C.
Author_xml – sequence: 1
  givenname: Thaís P.
  surname: Amadeu
  fullname: Amadeu, Thaís P.
  organization: From the Departamento de Histologia e Embriologiaa, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil, and GREFb, INSERM E0362, Université Victor Segalen - Bordeaux II, Bordeaux, France
– sequence: 2
  givenname: André S.
  surname: Braune
  fullname: Braune, André S.
  organization: From the Departamento de Histologia e Embriologiaa, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil, and GREFb, INSERM E0362, Université Victor Segalen - Bordeaux II, Bordeaux, France
– sequence: 3
  givenname: Luís C.
  surname: Porto
  fullname: Porto, Luís C.
  organization: From the Departamento de Histologia e Embriologiaa, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil, and GREFb, INSERM E0362, Université Victor Segalen - Bordeaux II, Bordeaux, France
– sequence: 4
  givenname: Alexis
  surname: Desmoulière
  fullname: Desmoulière, Alexis
  organization: From the Departamento de Histologia e Embriologiaa, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil, and GREFb, INSERM E0362, Université Victor Segalen - Bordeaux II, Bordeaux, France
– sequence: 5
  givenname: Andréa M. A.
  surname: Costa
  fullname: Costa, Andréa M. A.
  email: amacosta@uerj.br
  organization: From the Departamento de Histologia e Embriologiaa, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil, and GREFb, INSERM E0362, Université Victor Segalen - Bordeaux II, Bordeaux, France
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Berthod F, Germain L, Li H, Xu W, Damour O, Auger FA. Collagen fibril network and elastic system remodeling in a reconstructed skin transplanted on nude mice. Matrix Biol 2001;20: 463-73.
Weibel ER, Kistler GS, Scherle WF. Pratical stereological methods for morphometric cytology. J Cell Biol 1966;30: 23-38.DOI: 10.1083/jcb.30.1.23
Cotta-Pereira G, Rodrigo FG, David-Ferreira JF. The elastic system fibers. Adv Exp Med Biol 1977;79: 19-30.
Brown NJ, Smyth EA, Cross SS, Reed MW. Angiogenesis induction and regression in human surgical wounds. Wound Rep Reg 2002;10: 245-51.
Raghunath M, Bächi T, Meuli M, Altermatt S, Gobet R, Bruckner-Tuderman L, Steinmann B. Fibrillin and elastin expression in skin regenerating from cultured keratinocyte autografts: morphogenesis of microfibrils begins at the dermo-epidermal junction and precedes elastic fiber formation. J Invest Dermatol 1996; 106: 1090-5.DOI: 10.1111/1523-1747.ep12339373
Tsuji T, Sawabe M. Elastic fibers in scar tissue: scanning and transmission electron microscopic studies. J Cutan Pathol 1987;14: 106-13.
Kielty CM, Sherratt MJ, Shuttleworth CA. Elastic fibers. J Cell Sci 2002;115: 2817-28.
Williams G. The late phases of wound healing: histological and ultrastructural studies of collagen and elastic-tissue formation. J Pathol 1970;102: 61-8.
Meyer LJM, Russell SR, Russell JD, Trupin JS, Egbert BM, Shuster S, Stern R. Reduced hyaluronan in keloid tissue and cultured keloid fibroblasts. J Invest Dermatol 2000;114: 953-9.DOI: 10.1046/j.1523-1747.2000.00950.x
Swann DA, Garg HG, Hendry CJ, Hermann H, Siebert E, Sotman S, Stafford W. Isolation and partial characterization of dermatan sulfate proteoglycans from human post-burn scar tissues. Coll Relat Res 1988;8: 295-313.
Cowper SE, Su LD, Bhawan J, Robin HS, LeBoit PE. Nephrogenic fibrosing dermopathy. Am J Dermatopathol 2001;23: 383-93.
Kamath NV, Ormsby A, Bergfeld WF, House NS. A light microscopic and immunohistochemical evaluation of scars. J Cutan Pathol 2002;29: 27-32.DOI: 10.1034/j.1600-0560.2002.290105.x
Dalkowski A, Schuppan D, Orfanos CE, Zouboulis CC. Increased expression of tenascin C by keloids in vivo and in vitro. Br J Dermatol 1999;141: 50-6.DOI: 10.1046/j.1365-2133.1999.02920.x
Kielty CM, Shuttleworth CA. Microfibrillar elements of the dermal matrix. Microsc Res Tech 1997;38: 413-27.DOI: 10.1002/(SICI)1097-0029(19970815)38:4<413::AID-JEMT9>3.0.CO;2-J
Ghahary A, Shen YJ, Scott PG, Gong Y, Tredget EE. Enhanced expression of mRNA for transforming growth factor-beta, type I and type III procollagen in human post-burn hypertrophic scar tissues. J Lab Clin Med 1993;122: 465-73.
Raghunath M, Tschodrich-Rotter M, Sasaki T, Meuli M, Chu ML, Timpl R. Confocal laser scanning analysis of the association of fibulin-2 with fibrillin-1 and fibronectin define different stages of skin regeneration. J Invest Dermatol 1999;112: 97-101.DOI: 10.1046/j.1523-1747.1999.00483.x
Scott PG, Dodd CM, Tredget EE, Ghahary A, Rahemtulla F. Immunohistochemical localization of the proteoglycans decorin, biglycan and versican and transforming growth factor-beta in human post-burn hypertrophic and mature scars. Histopathology 1995;26: 423-31.
Sakai LY, Keene DR, Engvall E. Fibrillin, a new 350-kD glycoprotein, is a component of extracellular microfibrils. J Cell Biol 1986;103: 2499-509.DOI: 10.1083/jcb.103.6.2499
Dahlback K, Ljungquist A, Lofberg H, Dahlback B, Engvall E, Sakai LY. Fibrillin immunoreactive fibers constitute a unique network in the human dermis: immunohistochemical comparison of the distributions of fibrillin, vitronectin, amyloid P component, and orcein stainable structures in normal skin and elastosis. J Invest Dermatol 1990;94: 284-91.DOI: 10.1111/1523-1747.ep12874430
Craig RD. Collagen biosynthesis in normal human skin, normal and hypertrophic scar and keloid. Eur J Clin Invest 1975;5: 69-74.
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1987; 14
1989; 60
2004; 84
2001; 90
1970; 102
2000; 114
1994; 130
2002; 10
1999; 341
2002; 115
1999; 141
1996; 90
1988; 96
1996; 284
1998; 138
1998; 111
1999; 7
2001; 23
1966; 30
1990; 186
1996; 106
2001; 20
2003; 33
1993; 122
2001; 276
1986; 103
1976; 57
2002; 29
1997; 183
1995; 26
1988; 8
1997; 38
1999; 113
1977; 79
1999; 155
1999; 112
1975; 5
1990; 94
Kielty CM (e_1_2_6_14_2) 2002; 115
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Compton CC (e_1_2_6_30_2) 1989; 60
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Ghahary A (e_1_2_6_5_2) 1993; 122
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e_1_2_6_25_2
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Snippet Hypertrophic scars and keloids are two forms of excessive cutaneous scarring. Considering the importance of extracellular matrix elements in tissue repair, a...
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StartPage 169
SubjectTerms Adolescent
Adult
Case-Control Studies
Child
Cicatrix, Hypertrophic - metabolism
Cicatrix, Hypertrophic - pathology
Elastin - metabolism
Female
Fibrillin-1
Fibrillins
Humans
Keloid - metabolism
Keloid - pathology
Male
Microfilament Proteins - metabolism
Middle Aged
Skin - metabolism
Skin - pathology
Wound Healing - physiology
Title Fibrillin-1 and elastin are differentially expressed in hypertrophic scars and keloids
URI https://api.istex.fr/ark:/67375/WNG-V0RLDR8R-5/fulltext.pdf
https://cir.nii.ac.jp/crid/1572261550458546944
https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1067-1927.2004.012209.x
https://www.ncbi.nlm.nih.gov/pubmed/15086768
https://www.proquest.com/docview/71844839
Volume 12
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