Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response

BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship bet...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Memórias do Instituto Oswaldo Cruz Jg. 112; H. 5; S. 382 - 386
Hauptverfasser: Fonseca, Gabriela Rodrigues e, Santos, Sergio Vieira dos, Chieffi, Pedro Paulo, Paula, Fabiana Martins de, Gryschek, Ronaldo Cesar Borges, Lescano, Susana Angélica Zevallos
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01.05.2017
Fundação Oswaldo Cruz (FIOCRUZ)
Schlagworte:
Animals
Antibody Formation - immunology
BALB/c
Disease Models, Animal
ELISA
Immunoglobulin G - blood
Immunoglobulin G - immunology
larval recovery
Mice
Mice, Inbred BALB C
PARASITOLOGY
Short Communication
Toxocara canis
Toxocara canis - physiology
Toxocariasis - immunology
TROPICAL MEDICINE
Toxocara canis
larval recovery
BALB/c
ELISA
ISSN:0074-0276, 1678-8060, 1678-8060
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
AUTHORS’ CONTRIBUTION
GRF - Participated in all experiments, collaborated in the discussion and revision of the manuscript; SVS - performed the statistical analysis in all experiments, and participated in the discussion and revision of the manuscript; PPC - participated in the preparation of the project, discussion and revision of the manuscript; FMP - participated in the preparation of the project, discussion and revision of the manuscript; RCBG - participated in the composing, discussion and revision of the manuscript; SAZL - participated in the preparation of the project, in all experiments, composing, discussion and revision of the manuscript.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/0074-02760160341