Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery

BACKGROUND Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors presented evidence supporting the...

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Vydáno v:Cancer Ročník 121; číslo 18; s. 3230 - 3239
Hlavní autoři: Campanella, Claudia, Rappa, Francesca, Sciumè, Carmelo, Marino Gammazza, Antonella, Barone, Rosario, Bucchieri, Fabio, David, Sabrina, Curcurù, Giuseppe, Caruso Bavisotto, Celeste, Pitruzzella, Alessandro, Geraci, Girolamo, Modica, Giuseppe, Farina, Felicia, Zummo, Giovanni, Fais, Stefano, Conway de Macario, Everly, Macario, Alberto J.L., Cappello, Francesco
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 15.09.2015
Témata:
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Adenocarcinoma - surgery
Aged
Aged, 80 and over
Biomarkers, Tumor - analysis
Blotting, Western
Chaperonin 60 - analysis
Chaperonin 60 - metabolism
colon adenocarcinoma
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Colonic Neoplasms - surgery
Enzyme-Linked Immunosorbent Assay
exosomes
Exosomes - metabolism
Female
heat shock protein 60 (Hsp60)
Humans
Immunohistochemistry
macrophages
Male
Middle Aged
Mitochondrial Proteins - analysis
Mitochondrial Proteins - metabolism
natural killer cells
plasma cell membrane
Real-Time Polymerase Chain Reaction
theranostics
heat shock protein 60 (Hsp60)
plasma cell membrane
natural killer cells
macrophages
colon adenocarcinoma
exosomes
theranostics
ISSN:0008-543X, 1097-0142
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Shrnutí:BACKGROUND Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors presented evidence supporting the theory that Hsp60 has potential as a biomarker as well as a therapeutic target in patients with large bowel cancer. METHODS The authors studied a population of 97 subjects, including patients and controls. Immunomorphology, Western blot analysis, and quantitative real‐time polymerase chain reaction were performed on tissue specimens. Exosomes were isolated from blood and characterized by electron microscopy, biochemical tests, and Western blot analysis. RESULTS Hsp60 was found to be increased in cancerous tissue, in which it was localized in the tumor cell plasma membrane, and in the interstitium associated with cells of the immune system, in which it was associated with exosomes liberated by tumor cells and, as such, circulated in the blood. An interesting finding was that these parameters returned to normal shortly after tumor removal. CONCLUSIONS The data from the current study suggested that Hsp60 is a good candidate for theranostics applied to patients with large bowel carcinoma and encourage similar research among patients with other tumors in which Hsp60 has been implicated. Cancer 2015;121:3230–3239. © 2015 American Cancer Society. Heat shock protein 60 is a chaperonin involved in tumorigenesis but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors present evidence supporting the theory that heat shock protein 60 has potential as a biomarker as well as a therapeutic target in patients with colon cancer.
Bibliografie:The first 3 authors contributed equally to this work.
We thank Claudio Tripodo, MD, for the gift of anti‐CD57 antibody.
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ISSN:0008-543X
1097-0142
DOI:10.1002/cncr.29499