The Caenorhabditis elegans Transgenic Toolbox

The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, ne...

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Veröffentlicht in:Genetics (Austin) Jg. 212; H. 4; S. 959 - 990
Hauptverfasser: Nance, Jeremy, Frøkjær-Jensen, Christian
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Genetics Society of America 01.08.2019
Schlagworte:
Aging
Animals
Animals, Genetically Modified
Artificial chromosomes
BASIC (programming language)
Biology
Caenorhabditis elegans
Caenorhabditis elegans - genetics
Cloning
Control methods
CRISPR
CRISPR-Cas Systems
Deactivation
Gene expression
Genetic engineering
Genetic modification
Genetic Techniques
Genetics
Genome editing
Genomes
Genomics
Inactivation
Laboratories
Nematodes
Nervous system
Neurosciences
Proteins
Vectors (Biology)
Wormbook
Worms
transposon
C. elegans
transgenesis
CRISPR/Cas9
recombinases
WormBook
genetic engineering
ISSN:1943-2631, 0016-6731, 1943-2631
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the C. elegans transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in C. elegans, with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression.
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ISSN:1943-2631
0016-6731
1943-2631
DOI:10.1534/genetics.119.301506