Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa

In recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. Rousettus aegyptiacus (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This study focused on the...

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Veröffentlicht in:Viruses Jg. 15; H. 2; S. 498
Hauptverfasser: Rademan, Rochelle, Geldenhuys, Marike, Markotter, Wanda
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland MDPI AG 10.02.2023
MDPI
Schlagworte:
Adaptation
Africa, Southern
Amino Acids
Animals
Avian influenza viruses
Bats
birds
Chiroptera
Egyptian Rousette bat
Feces
genes
Genomes
H9N2
hosts
Humans
Identification and classification
Influenza
Influenza A
Influenza A virus
Influenza A Virus, H9N2 Subtype - genetics
Influenza, Human
Laboratories
mammals
monitoring
Mutation
RNA
RNA viruses
Rousettus
Rousettus aegyptiacus
Serology
South Africa
South Africa - epidemiology
Surveillance
Testing
Virulence
Viruses
Zoonoses
Africa, Southern
South Africa
Africa
Johannesburg South Africa
South Africa
surveillance
H9N2
Egyptian Rousette bat
influenza A virus
ISSN:1999-4915, 1999-4915
Online-Zugang:Volltext
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Zusammenfassung:In recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. Rousettus aegyptiacus (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This study focused on the molecular surveillance of a maternal colony in Limpopo, South Africa, between 2017–2018. A pan-influenza hemi-nested RT-PCR assay targeting the PB1 gene was established, and influenza A virus RNA was identified from one fecal sample out of 860 samples. Genome segments were recovered using segment-specific amplification combined with standard Sanger sequencing and Illumina unbiased sequencing. The identified influenza A virus was closely related to the H9N2 bat-influenza virus, confirming the circulation of this subtype among Egyptian fruit bat populations in Southern Africa. This bat H9N2 subtype contained amino acid residues associated with transmission and virulence in either mammalian or avian hosts, though it will likely require additional adaptations before spillover.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v15020498