Enhanced proliferation, attachment and osteopontin expression by porcine periodontal cells exposed to Emdogain

Emdogain ® (EMD) is an enamel matrix derivative extracted from developing porcine teeth with demonstrated periodontal regenerative potential. EMD has been shown to influence a number of properties of periodontal ligament cells including proliferation, cell attachment and matrix synthesis. To date, t...

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Bibliographic Details
Published in:Archives of oral biology Vol. 50; no. 12; pp. 1047 - 1054
Main Authors: Rincon, J.C., Xiao, Y., Young, W.G., Bartold, P.M.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.12.2005
Subjects:
Animals
Attachment
Cell Adhesion
Cell Proliferation
Cells, Cultured
Dental Enamel Proteins - pharmacology
DNA - biosynthesis
Emdogain
Epithelial cell rests of Malassez
Fibroblasts - drug effects
Fibroblasts - metabolism
Fibroblasts - pathology
Osteopontin
Periodontium - drug effects
Periodontium - metabolism
Periodontium - pathology
Proliferation
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
RT-PCR
Sialoglycoproteins - chemistry
Sialoglycoproteins - genetics
Sialoglycoproteins - metabolism
Swine
Osteopontin
Epithelial cell rests of Malassez
Attachment
Emdogain
Proliferation
RT-PCR
ISSN:0003-9969, 1879-1506
Online Access:Get full text
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Summary:Emdogain ® (EMD) is an enamel matrix derivative extracted from developing porcine teeth with demonstrated periodontal regenerative potential. EMD has been shown to influence a number of properties of periodontal ligament cells including proliferation, cell attachment and matrix synthesis. To date, the effect of EMD on the epithelial cell rests of Malassez (ERM) is unknown. In this study, periodontal ligament fibroblasts, ERM, alveolar bone cells and gingival fibroblasts were obtained from porcine periodontal ligament, alveolar bone and gingiva. This study investigated, in vitro, the effect of EMD at three concentrations on proliferation, cell attachment and expression of mRNA for two mineralised tissue-related proteins (osteopontin and bone sialoprotein). As for other periodontal cells, the ERM proliferative response was enhanced by EMD. Attachment assays revealed a highly significant increase for ERM and gingival fibroblasts after EMD treatment at all concentrations. This study has also shown that EMD stimulated expression of osteopontin mRNA by ERM and alveolar bone cells. The results from this study provide evidence that EMD enhanced cellular events related with proliferation, attachment and osteopontin mRNA expression by porcine periodontal cells, in a manner consistent with its role in periodontal regenerative therapy.
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ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2005.04.006