Identification of Histoplasma capsulatum from culture extracts by real-time PCR

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organis...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of clinical microbiology Jg. 41; H. 3; S. 1295
Hauptverfasser:	Martagon-Villamil, Jose, Shrestha, Nabin, Sholtis, Mary, Isada, Carlos M, Hall, Gerri S, Bryne, Terry, Lodge, Barbara A, Reller, L Barth, Procop, Gary W
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.03.2003
Schlagworte:	DNA, Fungal - analysis Histoplasma - genetics Histoplasma - isolation & purification Humans Microbiological Techniques Polymerase Chain Reaction - methods
ISSN:	0095-1137
Online-Zugang:	Weitere Angaben
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0095-1137
DOI:	10.1128/JCM.41.3.1295-1298.2003