Identification of Histoplasma capsulatum from culture extracts by real-time PCR

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organis...

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Bibliographic Details
Published in:Journal of clinical microbiology Vol. 41; no. 3; p. 1295
Main Authors: Martagon-Villamil, Jose, Shrestha, Nabin, Sholtis, Mary, Isada, Carlos M, Hall, Gerri S, Bryne, Terry, Lodge, Barbara A, Reller, L Barth, Procop, Gary W
Format: Journal Article
Language:English
Published: United States 01.03.2003
Subjects:
DNA, Fungal - analysis
Histoplasma - genetics
Histoplasma - isolation & purification
Humans
Microbiological Techniques
Polymerase Chain Reaction - methods
ISSN:0095-1137
Online Access:Get more information
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Summary:We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
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ISSN:0095-1137
DOI:10.1128/JCM.41.3.1295-1298.2003