Identification of Histoplasma capsulatum from culture extracts by real-time PCR

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organis...

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Vydáno v:Journal of clinical microbiology Ročník 41; číslo 3; s. 1295
Hlavní autoři: Martagon-Villamil, Jose, Shrestha, Nabin, Sholtis, Mary, Isada, Carlos M, Hall, Gerri S, Bryne, Terry, Lodge, Barbara A, Reller, L Barth, Procop, Gary W
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.03.2003
Témata:
DNA, Fungal - analysis
Histoplasma - genetics
Histoplasma - isolation & purification
Humans
Microbiological Techniques
Polymerase Chain Reaction - methods
ISSN:0095-1137
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Shrnutí:We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0095-1137
DOI:10.1128/JCM.41.3.1295-1298.2003